Seroatlas · Human Serome Atlas

CRYAB

Alpha-crystallin B chain

Also known as: CRYA2, CRYAB_HUMAN, HSPB5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P02511
Gene: CRYAB
Ensembl: ENSG00000109846
Chromosome: 11
Canonical length: 175 aa
Protein class: Cancer-related genes, Disease related genes, Human disease related genes, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Plasma membrane,Cytosol
Secretome location: Intracellular and membrane

OverviewNCBI Gene

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2019]

Canonical amino-acid sequenceUniProt

175 residues, UniProt reviewed canonical sequence.

>P02511|CRYAB
     1  MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW
    61  FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR
   121  KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CRYAB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 10,538 nTPM

Expression across tissuesHPA

Tissue

heart muscle: 10,538 nTPM
tongue: 8,773 nTPM
skeletal muscle: 7,430 nTPM
spinal cord: 4,674 nTPM
midbrain: 2,675 nTPM
kidney: 2,165 nTPM

Single-cell type

epididymal efferent duct absorptive cells: 3,659 nCPM
thymic myoid cells: 3,391 nCPM
schwann cells: 3,219 nCPM
esophageal apical cells: 2,670 nCPM
epididymal efferent duct ciliated cells: 2,186 nCPM
pancreatic duct cells: 1,898 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

white matter: 4,366 nTPM
medulla oblongata: 2,791 nTPM
basal ganglia: 2,511 nTPM
pons: 2,405 nTPM
cerebellum: 2,403 nTPM
spinal cord: 2,070 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CRYAB.

Disease | AllUniProt

Conditions CRYAB is implicated in, by any mechanism.

Myopathy, myofibrillar, 2A, adult-onset (MFM2A) MIM:608810
Cataract 16, multiple types (CTRCT16) MIM:613763
Myopathy, myofibrillar, 2B, infantile-onset (MFM2B) MIM:613869
Cardiomyopathy, dilated, 1II (CMD1II) MIM:615184

Disease | GeneticClinVar

18 pathogenic / likely-pathogenic of 349 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Dilated cardiomyopathy 1II
Cataract 16 multiple types
Myofibrillar myopathy 2
Cardiomyopathy, familial restrictive, 1
Developmental cataract

Disease | ImmuneIEDB

Conditions an epitope on CRYAB was assayed in.

multiple sclerosis B and T cell
autoimmune uveitis B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.43
gnomAD pLI: 0.02
gnomAD missense Z: 0.48
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CRYAB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CRYAB as an antibody target. Whether an autoantibody or antibody against CRYAB could matter depends on whether native CRYAB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CRYAB is annotated as secreted, so native CRYAB circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label CRYAB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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