Seroatlas · Human Serome Atlas

CENPU

Centromere protein U

Also known as: CENP-50, CENP-U, CENPU_HUMAN, KLIP1, MLF1IP, PBIP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q71F23
Gene
CENPU
Ensembl
ENSG00000151725
Chromosome
4
Canonical length
418 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Centriolar satellite

OverviewNCBI Gene

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

418 residues, UniProt reviewed canonical sequence.

>Q71F23|CENPU
     1  MAPRGRRRPR PHRSEGARRS KNTLERTHSM KDKAGQKCKP IDVFDFPDNS DVSSIGRLGE
    61  NEKDEETYET FDPPLHSTAI YADEEEFSKH CGLSLSSTPP GKEAKRSSDT SGNEASEIES
   121  VKISAKKPGR KLRPISDDSE SIEESDTRRK VKSAEKISTQ RHEVIRTTAS SELSEKPAES
   181  VTSKKTGPLS AQPSVEKENL AIESQSKTQK KGKISHDKRK KSRSKAIGSD TSDIVHIWCP
   241  EGMKTSDIKE LNIVLPEFEK THLEHQQRIE SKVCKAAIAT FYVNVKEQFI KMLKESQMLT
   301  NLKRKNAKMI SDIEKKRQRM IEVQDELLRL EPQLKQLQTK YDELKERKSS LRNAAYFLSN
   361  LKQLYQDYSD VQAQEPNVKE TYDSSSLPAL LFKARTLLGA ESHLRNINHQ LEKLLDQG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CENPU can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.62
Highest tissue expression
59 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 59 nTPM
  • testis: 28 nTPM
  • lymph node: 15 nTPM
  • skin: 14 nTPM
  • tonsil: 13 nTPM
  • thymus: 12 nTPM

Single-cell type

  • late spermatids: 1,614 nCPM
  • late primary spermatocytes: 809 nCPM
  • early primary spermatocytes: 469 nCPM
  • early spermatids: 417 nCPM
  • oocytes: 240 nCPM
  • erythrocyte progenitors: 230 nCPM

Immune cell

  • basophil: 21 nTPM
  • eosinophil: 14 nTPM
  • NK-cell: 3.6 nTPM
  • non-classical monocyte: 2.8 nTPM
  • T-reg: 2.5 nTPM
  • intermediate monocyte: 2 nTPM

Brain region

  • cerebellum: 1.8 nTPM
  • pons: 1.8 nTPM
  • white matter: 1.6 nTPM
  • thalamus: 1.4 nTPM
  • cerebral cortex: 1.3 nTPM
  • basal ganglia: 1.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.98
gnomAD pLI
0
gnomAD missense Z
-0.46
DepMap mean gene effect
-0.14
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Centromere protein U
  • CENP-A nucleosome associated complex (NAC) subunit

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CENPU in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CENPU as an antibody target. Whether an autoantibody or antibody against CENPU could matter depends on whether native CENPU is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CENPU is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CENPU as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CENPU. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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