Seroatlas · Human Serome Atlas

MLF1

Myeloid leukemia factor 1

Also known as: MLF1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P58340
Gene
MLF1
Ensembl
ENSG00000178053
Chromosome
3
Canonical length
268 aa
Protein class
Cancer-related genes, Disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles,Plasma membrane,Focal adhesion sites,Centrosome,Basal body,Cytosol,Mid piece,Principal piece,End piece

OverviewNCBI Gene

This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Canonical amino-acid sequenceUniProt

268 residues, UniProt reviewed canonical sequence.

>P58340|MLF1
     1  MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG
    61  EDSLTHTDVS SFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP
   121  PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI HDRAHVIKKS KNKKTGDEEV
   181  NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS
   241  PAIEHGRRSN VLGDKLHIKG SSVKSNKK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MLF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.46
Highest tissue expression
340 nTPM

Expression across tissuesHPA

Tissue

  • testis: 340 nTPM
  • skeletal muscle: 171 nTPM
  • heart muscle: 118 nTPM
  • tongue: 100 nTPM
  • choroid plexus: 91 nTPM
  • fallopian tube: 64 nTPM

Single-cell type

  • late spermatids: 19,792 nCPM
  • early spermatids: 5,555 nCPM
  • late primary spermatocytes: 2,733 nCPM
  • early primary spermatocytes: 484 nCPM
  • fallopian tube ciliated cells: 467 nCPM
  • epididymal efferent duct ciliated cells: 434 nCPM

Immune cell

  • T-reg: 8.8 nTPM
  • memory CD4 T-cell: 6.9 nTPM
  • memory CD8 T-cell: 6.9 nTPM
  • naive CD8 T-cell: 3.3 nTPM
  • gdT-cell: 2.8 nTPM
  • total PBMC: 2 nTPM

Brain region

  • choroid plexus: 53 nTPM
  • medulla oblongata: 34 nTPM
  • midbrain: 33 nTPM
  • white matter: 30 nTPM
  • spinal cord: 26 nTPM
  • basal ganglia: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.22
gnomAD pLI
0
gnomAD missense Z
0.59
DepMap mean gene effect
0.01
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MLF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MLF1 as an antibody target. Whether an autoantibody or antibody against MLF1 could matter depends on whether native MLF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MLF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MLF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MLF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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