CENPE

Protein identityUniProt · HPA

UniProt accession

Q02224

Gene

CENPE

Ensembl

ENSG00000138778

Chromosome

4

Canonical length

2701 aa

Protein class

Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Microtubules,Cytokinetic bridge,Mitotic spindle

OverviewNCBI Gene

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

Canonical amino-acid sequenceUniProt

2701 residues, UniProt reviewed canonical sequence.

>Q02224|CENPE
     1  MAEEGAVAVC VRVRPLNSRE ESLGETAQVY WKTDNNVIYQ VDGSKSFNFD RVFHGNETTK
    61  NVYEEIAAPI IDSAIQGYNG TIFAYGQTAS GKTYTMMGSE DHLGVIPRAI HDIFQKIKKF
   121  PDREFLLRVS YMEIYNETIT DLLCGTQKMK PLIIREDVNR NVYVADLTEE VVYTSEMALK
   181  WITKGEKSRH YGETKMNQRS SRSHTIFRMI LESREKGEPS NCEGSVKVSH LNLVDLAGSE
   241  RAAQTGAAGV RLKEGCNINR SLFILGQVIK KLSDGQVGGF INYRDSKLTR ILQNSLGGNA
   301  KTRIICTITP VSFDETLTAL QFASTAKYMK NTPYVNEVST DEALLKRYRK EIMDLKKQLE
   361  EVSLETRAQA MEKDQLAQLL EEKDLLQKVQ NEKIENLTRM LVTSSSLTLQ QELKAKRKRR
   421  VTWCLGKINK MKNSNYADQF NIPTNITTKT HKLSINLLRE IDESVCSESD VFSNTLDTLS
   481  EIEWNPATKL LNQENIESEL NSLRADYDNL VLDYEQLRTE KEEMELKLKE KNDLDEFEAL
   541  ERKTKKDQEM QLIHEISNLK NLVKHAEVYN QDLENELSSK VELLREKEDQ IKKLQEYIDS
   601  QKLENIKMDL SYSLESIEDP KQMKQTLFDA ETVALDAKRE SAFLRSENLE LKEKMKELAT
   661  TYKQMENDIQ LYQSQLEAKK KMQVDLEKEL QSAFNEITKL TSLIDGKVPK DLLCNLELEG
   721  KITDLQKELN KEVEENEALR EEVILLSELK SLPSEVERLR KEIQDKSEEL HIITSEKDKL
   781  FSEVVHKESR VQGLLEEIGK TKDDLATTQS NYKSTDQEFQ NFKTLHMDFE QKYKMVLEEN
   841  ERMNQEIVNL SKEAQKFDSS LGALKTELSY KTQELQEKTR EVQERLNEME QLKEQLENRD
   901  STLQTVEREK TLITEKLQQT LEEVKTLTQE KDDLKQLQES LQIERDQLKS DIHDTVNMNI
   961  DTQEQLRNAL ESLKQHQETI NTLKSKISEE VSRNLHMEEN TGETKDEFQQ KMVGIDKKQD
  1021  LEAKNTQTLT ADVKDNEIIE QQRKIFSLIQ EKNELQQMLE SVIAEKEQLK TDLKENIEMT
  1081  IENQEELRLL GDELKKQQEI VAQEKNHAIK KEGELSRTCD RLAEVEEKLK EKSQQLQEKQ
  1141  QQLLNVQEEM SEMQKKINEI ENLKNELKNK ELTLEHMETE RLELAQKLNE NYEEVKSITK
  1201  ERKVLKELQK SFETERDHLR GYIREIEATG LQTKEELKIA HIHLKEHQET IDELRRSVSE
  1261  KTAQIINTQD LEKSHTKLQE EIPVLHEEQE LLPNVKEVSE TQETMNELEL LTEQSTTKDS
  1321  TTLARIEMER LRLNEKFQES QEEIKSLTKE RDNLKTIKEA LEVKHDQLKE HIRETLAKIQ
  1381  ESQSKQEQSL NMKEKDNETT KIVSEMEQFK PKDSALLRIE IEMLGLSKRL QESHDEMKSV
  1441  AKEKDDLQRL QEVLQSESDQ LKENIKEIVA KHLETEEELK VAHCCLKEQE ETINELRVNL
  1501  SEKETEISTI QKQLEAINDK LQNKIQEIYE KEEQFNIKQI SEVQEKVNEL KQFKEHRKAK
  1561  DSALQSIESK MLELTNRLQE SQEEIQIMIK EKEEMKRVQE ALQIERDQLK ENTKEIVAKM
  1621  KESQEKEYQF LKMTAVNETQ EKMCEIEHLK EQFETQKLNL ENIETENIRL TQILHENLEE
  1681  MRSVTKERDD LRSVEETLKV ERDQLKENLR ETITRDLEKQ EELKIVHMHL KEHQETIDKL
  1741  RGIVSEKTNE ISNMQKDLEH SNDALKAQDL KIQEELRIAH MHLKEQQETI DKLRGIVSEK
  1801  TDKLSNMQKD LENSNAKLQE KIQELKANEH QLITLKKDVN ETQKKVSEME QLKKQIKDQS
  1861  LTLSKLEIEN LNLAQKLHEN LEEMKSVMKE RDNLRRVEET LKLERDQLKE SLQETKARDL
  1921  EIQQELKTAR MLSKEHKETV DKLREKISEK TIQISDIQKD LDKSKDELQK KIQELQKKEL
  1981  QLLRVKEDVN MSHKKINEME QLKKQFEAQN LSMQSVRMDN FQLTKKLHES LEEIRIVAKE
  2041  RDELRRIKES LKMERDQFIA TLREMIARDR QNHQVKPEKR LLSDGQQHLT ESLREKCSRI
  2101  KELLKRYSEM DDHYECLNRL SLDLEKEIEF QKELSMRVKA NLSLPYLQTK HIEKLFTANQ
  2161  RCSMEFHRIM KKLKYVLSYV TKIKEEQHES INKFEMDFID EVEKQKELLI KIQHLQQDCD
  2221  VPSRELRDLK LNQNMDLHIE EILKDFSESE FPSIKTEFQQ VLSNRKEMTQ FLEEWLNTRF
  2281  DIEKLKNGIQ KENDRICQVN NFFNNRIIAI MNESTEFEER SATISKEWEQ DLKSLKEKNE
  2341  KLFKNYQTLK TSLASGAQVN PTTQDNKNPH VTSRATQLTT EKIRELENSL HEAKESAMHK
  2401  ESKIIKMQKE LEVTNDIIAK LQAKVHESNK CLEKTKETIQ VLQDKVALGA KPYKEEIEDL
  2461  KMKLVKIDLE KMKNAKEFEK EISATKATVE YQKEVIRLLR ENLRRSQQAQ DTSVISEHTD
  2521  PQPSNKPLTC GGGSGIVQNT KALILKSEHI RLEKEISKLK QQNEQLIKQK NELLSNNQHL
  2581  SNEVKTWKER TLKREAHKQV TCENSPKSPK VTGTASKKKQ ITPSQCKERN LQDPVPKESP
  2641  KSCFFDSRSK SLPSPHPVRY FDNSSLGLCP EVQNAGAESV DSQPGPWHAS SGKDVPECKT
  2701  Q

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CENPE can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0

Highest tissue expression

9.4 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CENPE.

Disease | AllUniProt

Conditions CENPE is implicated in, by any mechanism.

• Microcephaly 13, primary, autosomal recessive (MCPH13) MIM:616051

Disease | GeneticClinVar

5 pathogenic / likely-pathogenic of 590 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Microcephaly 13, primary, autosomal recessive

Disease | ImmuneIEDB

Conditions an epitope on CENPE was assayed in.

• systemic scleroderma B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.31

gnomAD pLI

0.24

gnomAD missense Z

1.27

DepMap mean gene effect

-0.96

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• attachment of mitotic spindle microtubules to kinetochore
• cell division
• chromosome segregation
• kinetochore assembly
• metaphase chromosome alignment
• microtubule-based movement
• mitotic cell cycle
• mitotic chromosome movement towards spindle pole
• mitotic metaphase chromosome alignment
• mitotic spindle organization
• positive regulation of protein kinase activity
• regulation of mitotic metaphase/anaphase transition
• lateral attachment of mitotic spindle microtubules to kinetochore
• microtubule plus-end directed mitotic chromosome migration

Molecular functions

• ATP binding
• kinetochore binding
• microtubule binding
• microtubule motor activity

Cellular components

• chromosome
• chromosome, centromeric region
• condensed chromosome, centromeric region
• cytosol
• intercellular bridge
• kinetochore
• kinetochore microtubule
• membrane
• microtubule
• microtubule cytoskeleton
• midbody
• mitotic spindle
• mitotic spindle midzone
• nucleus
• spindle midzone

Protein domainsUniProt · Pfam · InterPro

• Kinesin motor domain
• Kinesin motor domain, conserved site
• P-loop containing nucleoside triphosphate hydrolase
• Kinesin-like protein
• Kinesin motor domain superfamily
• Kinesin motor domain

KeywordsUniProt

• ATP-binding
• Cell cycle
• Cell division
• Centromere
• Chromosome
• Coiled coil
• Cytoplasm
• Cytoskeleton
• Developmental protein
• Kinetochore
• Lipoprotein
• Methylation
• Microtubule
• Mitosis
• Motor protein
• Nucleotide-binding
• Phosphoprotein
• Prenylation
• Primary microcephaly
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of CENPE in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• BUB1B
• CENPF
• CTCF
• KIF18A
• NUF2
• PRC1
• SEPT7
• SKAP
• TRAPPC12
• KNSTRN
• SEPTIN7
• CLASP2