Seroatlas · Human Serome Atlas

TRIM65

E3 ubiquitin-protein ligase TRIM65

Also known as: TRI65_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6PJ69
Gene
TRIM65
Ensembl
ENSG00000141569
Chromosome
17
Canonical length
517 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

Enables ubiquitin protein ligase activity. Involved in negative regulation of inflammatory response; positive regulation of metabolic process; and protein polyubiquitination. Located in cytosol and nucleoplasm. Is active in cytoplasm. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

517 residues, UniProt reviewed canonical sequence.

>Q6PJ69|TRIM65
     1  MAAQLLEEKL TCAICLGLYQ DPVTLPCGHN FCGACIRDWW DRCGKACPEC REPFPDGAEL
    61  RRNVALSGVL EVVRAGPARD PGPDPGPGPD PAARCPRHGR PLELFCRTEG RCVCSVCTVR
   121  ECRLHERALL DAERLKREAQ LRASLEVTQQ QATQAEGQLL ELRKQSSQIQ NSACILASWV
   181  SGKFSSLLQA LEIQHTTALR SIEVAKTQAL AQARDEEQRL RVHLEAVARH GCRIRELLEQ
   241  VDEQTFLQES QLLQPPGPLG PLTPLQWDED QQLGDLKQLL SRLCGLLLEE GSHPGAPAKP
   301  VDLAPVEAPG PLAPVPSTVC PLRRKLWQNY RNLTFDPVSA NRHFYLSRQD QQVKHCRQSR
   361  GPGGPGSFEL WQVQCAQSFQ AGHHYWEVRA SDHSVTLGVS YPQLPRCRLG PHTDNIGRGP
   421  CSWGLCVQED SLQAWHNGEA QRLPGVSGRL LGMDLDLASG CLTFYSLEPQ TQPLYTFHAL
   481  FNQPLTPVFW LLEGRTLTLC HQPGAVFPLG PQEEVLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TRIM65 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
20 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 20 nTPM
  • spleen: 16 nTPM
  • pancreas: 15 nTPM
  • endometrium: 14 nTPM
  • cervix: 14 nTPM
  • skin: 14 nTPM

Single-cell type

  • alveolar cells type 1: 24 nCPM
  • myonuclei: 23 nCPM
  • paneth cells: 23 nCPM
  • retinal pigment epithelial cells: 21 nCPM
  • gonadotrophs: 21 nCPM
  • retinal bipolar cells: 21 nCPM

Immune cell

  • MAIT T-cell: 2.2 nTPM
  • naive B-cell: 2 nTPM
  • NK-cell: 2 nTPM
  • gdT-cell: 1.9 nTPM
  • memory CD8 T-cell: 1.9 nTPM
  • eosinophil: 1.8 nTPM

Brain region

  • white matter: 27 nTPM
  • cerebellum: 24 nTPM
  • medulla oblongata: 24 nTPM
  • basal ganglia: 24 nTPM
  • midbrain: 24 nTPM
  • cerebral cortex: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.84
gnomAD pLI
0
gnomAD missense Z
0.41
DepMap mean gene effect
-0.17
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TRIM65 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TRIM65 as an antibody target. Whether an autoantibody or antibody against TRIM65 could matter depends on whether native TRIM65 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TRIM65 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TRIM65 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TRIM65. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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