Seroatlas · Human Serome Atlas

PRKAA1

5'-AMP-activated protein kinase catalytic subunit alpha-1

Also known as: AAPK1_HUMAN, AMPKa1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q13131
Gene
PRKAA1
Ensembl
ENSG00000132356
Chromosome
5
Canonical length
559 aa
Protein class
Enzymes, FDA approved drug targets, Predicted intracellular proteins
Subcellular location
Nuclear speckles,Primary cilium,Basal body,Cytosol

OverviewNCBI Gene

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

559 residues, UniProt reviewed canonical sequence.

>Q13131|PRKAA1
     1  MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG HKVAVKILNR
    61  QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI FMVMEYVSGG ELFDYICKNG
   121  RLDEKESRRL FQQILSGVDY CHRHMVVHRD LKPENVLLDA HMNAKIADFG LSNMMSDGEF
   181  LRTSCGSPNY AAPEVISGRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG
   241  IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID
   301  DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD FYLATSPPDS
   361  FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ GVRKAKWHLG IRSQSRPNDI
   421  MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE
   481  ITEAKSGTAT PQRSGSVSNY RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG
   541  SHTIEFFEMC ANLIKILAQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRKAA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
49 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 49 nTPM
  • liver: 45 nTPM
  • testis: 38 nTPM
  • duodenum: 33 nTPM
  • rectum: 32 nTPM
  • stomach: 31 nTPM

Single-cell type

  • late spermatids: 708 nCPM
  • neutrophil progenitors: 280 nCPM
  • early spermatids: 277 nCPM
  • late primary spermatocytes: 263 nCPM
  • neutrophils: 144 nCPM
  • parietal cells: 138 nCPM

Immune cell

  • basophil: 17 nTPM
  • neutrophil: 15 nTPM
  • eosinophil: 13 nTPM
  • T-reg: 12 nTPM
  • MAIT T-cell: 11 nTPM
  • NK-cell: 11 nTPM

Brain region

  • choroid plexus: 27 nTPM
  • pons: 26 nTPM
  • medulla oblongata: 25 nTPM
  • hypothalamus: 24 nTPM
  • spinal cord: 23 nTPM
  • cerebellum: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.48
gnomAD pLI
0.14
gnomAD missense Z
2.49
DepMap mean gene effect
0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRKAA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRKAA1 as an antibody target. Whether an autoantibody or antibody against PRKAA1 could matter depends on whether native PRKAA1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRKAA1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRKAA1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PRKAA1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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