Seroatlas · Human Serome Atlas

PRKAB1

5'-AMP-activated protein kinase subunit beta-1

Also known as: AAKB1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y478
Gene: PRKAB1
Ensembl: ENSG00000111725
Chromosome: 12
Canonical length: 270 aa
Protein class: FDA approved drug targets, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Primary cilium transition zone,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

270 residues, UniProt reviewed canonical sequence.

>Q9Y478|PRKAB1
     1  MGNTSSERAA LERHGGHKTP RRDSSGGTKD GDRPKILMDS PEDADLFHSE EIKAPEKEEF
    61  LAWQHDLEVN DKAPAQARPT VFRWTGGGKE VYLSGSFNNW SKLPLTRSHN NFVAILDLPE
   121  GEHQYKFFVD GQWTHDPSEP IVTSQLGTVN NIIQVKKTDF EVFDALMVDS QKCSDVSELS
   181  SSPPGPYHQE PYVCKPEERF RAPPILPPHL LQVILNKDTG ISCDPALLPE PNHVMLNHLY
   241  ALSIKDGVMV LSATHRYKKK YVTTLLYKPI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRKAB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.5
Highest tissue expression: 50 nTPM

Expression across tissuesHPA

Tissue

kidney: 50 nTPM
bone marrow: 42 nTPM
colon: 33 nTPM
pancreas: 32 nTPM
rectum: 31 nTPM
duodenum: 31 nTPM

Single-cell type

esophageal apical cells: 85 nCPM
renal collecting duct intercalated cells: 55 nCPM
distal convoluted tubule cells: 53 nCPM
salivary duct cells: 50 nCPM
proximal tubule cells: 43 nCPM
breast lactating cells: 43 nCPM

Immune cell

eosinophil: 71 nTPM
basophil: 51 nTPM
memory B-cell: 47 nTPM
NK-cell: 42 nTPM
naive B-cell: 40 nTPM
non-classical monocyte: 39 nTPM

Brain region

white matter: 26 nTPM
medulla oblongata: 25 nTPM
pons: 24 nTPM
midbrain: 22 nTPM
thalamus: 22 nTPM
hypothalamus: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.84
gnomAD pLI: 0.01
gnomAD missense Z: 1.25
DepMap mean gene effect: 0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

protein kinase binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRKAB1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRKAB1 as an antibody target. Whether an autoantibody or antibody against PRKAB1 could matter depends on whether native PRKAB1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRKAB1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRKAB1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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