Seroatlas · Human Serome Atlas

PRKAG1

5'-AMP-activated protein kinase subunit gamma-1

Also known as: AAKG1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P54619
Gene
PRKAG1
Ensembl
ENSG00000181929
Chromosome
12
Canonical length
331 aa
Protein class
FDA approved drug targets, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies,Basal body,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

331 residues, UniProt reviewed canonical sequence.

>P54619|PRKAG1
     1  METVISSDSS PAVENEHPQE TPESNNSVYT SFMKSHRCYD LIPTSSKLVV FDTSLQVKKA
    61  FFALVTNGVR AAPLWDSKKQ SFVGMLTITD FINILHRYYK SALVQIYELE EHKIETWREV
   121  YLQDSFKPLV CISPNASLFD AVSSLIRNKI HRLPVIDPES GNTLYILTHK RILKFLKLFI
   181  TEFPKPEFMS KSLEELQIGT YANIAMVRTT TPVYVALGIF VQHRVSALPV VDEKGRVVDI
   241  YSKFDVINLA AEKTYNNLDV SVTKALQHRS HYFEGVLKCY LHETLETIIN RLVEAEVHRL
   301  VVVDENDVVK GIVSLSDILQ ALVLTGGEKK P

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRKAG1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
114 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 114 nTPM
  • tongue: 105 nTPM
  • heart muscle: 76 nTPM
  • epididymis: 55 nTPM
  • parathyroid gland: 55 nTPM
  • adrenal gland: 53 nTPM

Single-cell type

  • choroid plexus epithelial cells: 41 nCPM
  • renal collecting duct intercalated cells: 38 nCPM
  • other brain neurons: 37 nCPM
  • microglia: 35 nCPM
  • bergmann glia: 35 nCPM
  • podocytes: 32 nCPM

Immune cell

  • basophil: 87 nTPM
  • total PBMC: 73 nTPM
  • NK-cell: 72 nTPM
  • neutrophil: 71 nTPM
  • myeloid DC: 69 nTPM
  • T-reg: 67 nTPM

Brain region

  • cerebral cortex: 77 nTPM
  • choroid plexus: 74 nTPM
  • basal ganglia: 72 nTPM
  • cerebellum: 68 nTPM
  • white matter: 68 nTPM
  • hypothalamus: 67 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.8
gnomAD pLI
0
gnomAD missense Z
1.85
DepMap mean gene effect
-0.3
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRKAG1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRKAG1 as an antibody target. Whether an autoantibody or antibody against PRKAG1 could matter depends on whether native PRKAG1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRKAG1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRKAG1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PRKAG1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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