Seroatlas · Human Serome Atlas

PLK4

Serine/threonine-protein kinase PLK4

Also known as: PLK4_HUMAN, Sak, STK18

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O00444
Gene: PLK4
Ensembl: ENSG00000142731
Chromosome: 4
Canonical length: 970 aa
Protein class: Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location: Centrosome,Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Canonical amino-acid sequenceUniProt

970 residues, UniProt reviewed canonical sequence.

>O00444|PLK4
     1  MATCIGEKIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE
    61  VKIHCQLKHP SILELYNYFE DSNYVYLVLE MCHNGEMNRY LKNRVKPFSE NEARHFMHQI
   121  ITGMLYLHSH GILHRDLTLS NLLLTRNMNI KIADFGLATQ LKMPHEKHYT LCGTPNYISP
   181  EIATRSAHGL ESDVWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PSFLSIEAKD
   241  LIHQLLRRNP ADRLSLSSVL DHPFMSRNSS TKSKDLGTVE DSIDSGHATI STAITASSST
   301  SISGSLFDKR RLLIGQPLPN KMTVFPKNKS STDFSSSGDG NSFYTQWGNQ ETSNSGRGRV
   361  IQDAEERPHS RYLRRAYSSD RSGTSNSQSQ AKTYTMERCH SAEMLSVSKR SGGGENEERY
   421  SPTDNNANIF NFFKEKTSSS SGSFERPDNN QALSNHLCPG KTPFPFADPT PQTETVQQWF
   481  GNLQINAHLR KTTEYDSISP NRDFQGHPDL QKDTSKNAWT DTKVKKNSDA SDNAHSVKQQ
   541  NTMKYMTALH SKPEIIQQEC VFGSDPLSEQ SKTRGMEPPW GYQNRTLRSI TSPLVAHRLK
   601  PIRQKTKKAV VSILDSEEVC VELVKEYASQ EYVKEVLQIS SDGNTITIYY PNGGRGFPLA
   661  DRPPSPTDNI SRYSFDNLPE KYWRKYQYAS RFVQLVRSKS PKITYFTRYA KCILMENSPG
   721  ADFEVWFYDG VKIHKTEDFI QVIEKTGKSY TLKSESEVNS LKEEIKMYMD HANEGHRICL
   781  ALESIISEEE RKTRSAPFFP IIIGRKPGST SSPKALSPPP SVDSNYPTRE RASFNRMVMH
   841  SAASPTQAPI LNPSMVTNEG LGLTTTASGT DISSNSLKDC LPKSAQLLKS VFVKNVGWAT
   901  QLTSGAVWVQ FNDGSQLVVQ AGVSSISYTS PNGQTTRYGE NEKLPDYIKQ KLQCLSSILL
   961  MFSNPTPNFH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PLK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 21 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 21 nTPM
testis: 21 nTPM
thymus: 13 nTPM
lymph node: 6.9 nTPM
tonsil: 6.9 nTPM
appendix: 4.6 nTPM

Single-cell type

late spermatids: 139 nCPM
late primary spermatocytes: 104 nCPM
respiratory deuterosomal cells: 70 nCPM
early spermatids: 63 nCPM
erythrocyte progenitors: 62 nCPM
monocyte progenitors: 47 nCPM

Immune cell

T-reg: 1.2 nTPM
naive CD8 T-cell: 0.6 nTPM
memory CD4 T-cell: 0.5 nTPM
memory CD8 T-cell: 0.5 nTPM
eosinophil: 0.4 nTPM
gdT-cell: 0.4 nTPM

Brain region

thalamus: 1.5 nTPM
hypothalamus: 1.4 nTPM
basal ganglia: 1.3 nTPM
cerebral cortex: 1.3 nTPM
pons: 1.3 nTPM
hippocampal formation: 1.1 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PLK4.

Disease | AllUniProt

Conditions PLK4 is implicated in, by any mechanism.

Microcephaly and chorioretinopathy, autosomal recessive, 2 (MCCRP2) MIM:616171

Disease | GeneticClinVar

34 pathogenic / likely-pathogenic of 726 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Microcephaly and chorioretinopathy 2
PLK4-related microcephaly and growth failure with or without ocular features

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.57
gnomAD pLI: 0
gnomAD missense Z: 0.8
DepMap mean gene effect: -1.17
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Protein kinase domain
POLO box domain
Tyrosine-protein kinase, active site
Protein kinase-like domain superfamily
Protein kinase, ATP binding site
Protein kinase domain
Plk4, C-terminal polo-box domain
Plk4, second cryptic polo-box domain
Plk4, first cryptic polo-box domain
Serine/threonine-protein kinase, first cryptic polo-box domain superfamily
Plk4-like, second cryptic polo-box domain superfamily
Polo-like Kinase 4 Polo Box 1
Polo-like Kinase 4 Polo Box 2

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PLK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PLK4 as an antibody target. Whether an autoantibody or antibody against PLK4 could matter depends on whether native PLK4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PLK4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PLK4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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