Seroatlas · Human Serome Atlas

NOL8

Nucleolar protein 8

Also known as: C9orf34, FLJ20736, NOL8_HUMAN, Nop132

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q76FK4
Gene: NOL8
Ensembl: ENSG00000198000
Chromosome: 9
Canonical length: 1167 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli,Mitotic chromosome

OverviewNCBI Gene

NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 [PubMed 14660641]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

1167 residues, UniProt reviewed canonical sequence.

>Q76FK4|NOL8
     1  MKVNRETKRL YVGGLSQDIS EADLQNQFSR FGEVSDVEII TRKDDQGNPQ KVFAYINISV
    61  AEADLKKCMS VLNKTKWKGG TLQIQLAKES FLHRLAQERE AAKAKKEEST TGNANLLEKT
   121  GGVDFHMKAV PGTEVPGHKN WVVSKFGRVL PVLHLKNQHK RKIIKYDPSK YCHNLKKIGE
   181  DFSNTIPISS LTWELEGGND PMSKKRRGEF SDFHGPPKKI IKVQKDESST GSLAMSTRPR
   241  RVIERPPLTQ QQAAQKRTCD SITPSKSSPV PVSDTQKLKN LPFKTSGLET AKKRNSISDD
   301  DTDSEDELRM MIAKEENLQR TTQPSINESE SDPFEVVRDD FKSGVHKLHS LIGLGIKNRV
   361  SCHDSDDDIM RNDREYDSGD TDEIIAMKKN VAKVKNSTEF SQMEKSTKKT SFKNRENCEL
   421  SDHCIKLQKR KSNVESALSH GLKSLNRKSP SHSSSSEDAD SASELADSEG GEEYNAMMKN
   481  CLRVNLTLAD LEQLAGSDLK VPNEDTKSDG PETTTQCKFD RGSKSPKTPT GLRRGRQCIR
   541  PAEIVASLLE GEENTCGKQK PKENNLKPKF QAFKGVGCLY EKESMKKSLK DSVASNNKDQ
   601  NSMKHEDPSI ISMEDGSPYV NGSLGEVTPC QHAKKANGPN YIQPQKRQTT FESQDRKAVS
   661  PSSSEKRSKN PISRPLEGKK SLSLSAKTHN IGFDKDSCHS TTKTEASQEE RSDSSGLTSL
   721  KKSPKVSSKD TREIKTDFSL SISNSSDVSA KDKHAEDNEK RLAALEARQK AKEVQKKLVH
   781  NALANLDGHP EDKPTHIIFG SDSECETEET STQEQSHPGE EWVKESMGKT SGKLFDSSDD
   841  DESDSEDDSN RFKIKPQFEG RAGQKLMDLQ SHFGTDDRFR MDSRFLETDS EEEQEEVNEK
   901  KTAEEEELAE EKKKALNVVQ SVLQINLSNS TNRGSVAAKK FKDIIHYDPT KQDHATYERK
   961  RDDKPKESKA KRKKKREEAE KLPEVSKEMY YNIAMDLKEI FQTTKYTSEK EEGTPWNEDC
  1021  GKEKPEEIQD PAALTSDAEQ PSGFTFSFFD SDTKDIKEET YRVETVKPGK IVWQEDPRLQ
  1081  DSSSEEEDVT EETDHRNSSP GEASLLEKET TRFFFFSKND ERLQGSDLFW RGVGSNMSRN
  1141  SWEARTTNLR MDCRKKHKDA KRKMKPK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOL8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.62
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

parathyroid gland: 12 nTPM
tonsil: 12 nTPM
thymus: 12 nTPM
lymph node: 11 nTPM
thyroid gland: 9.8 nTPM
ovary: 9.6 nTPM

Single-cell type

megakaryocyte-erythroid progenitors: 113 nCPM
early primary spermatocytes: 99 nCPM
erythrocyte progenitors: 89 nCPM
megakaryocyte progenitors: 81 nCPM
hematopoietic stem cells: 55 nCPM
pdcs: 55 nCPM

Immune cell

basophil: 19 nTPM
non-classical monocyte: 16 nTPM
MAIT T-cell: 16 nTPM
T-reg: 14 nTPM
naive CD4 T-cell: 13 nTPM
NK-cell: 13 nTPM

Brain region

white matter: 14 nTPM
thalamus: 12 nTPM
basal ganglia: 12 nTPM
medulla oblongata: 12 nTPM
pons: 11 nTPM
midbrain: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.83
gnomAD pLI: 0
gnomAD missense Z: 0.44
DepMap mean gene effect: -0.79
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

RNA recognition motif domain
Nucleotide-binding alpha-beta plait domain superfamily
RNA-binding domain superfamily
RNA recognition motif
Nucleolar protein 8, RNA recognition motif

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NOL8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NOL8 as an antibody target. Whether an autoantibody or antibody against NOL8 could matter depends on whether native NOL8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NOL8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NOL8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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