DDX47

Protein identityUniProt · HPA

UniProt accession

Q9H0S4

Gene

DDX47

Ensembl

ENSG00000213782

Chromosome

12

Canonical length

455 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoli

OverviewNCBI Gene

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

455 residues, UniProt reviewed canonical sequence.

>Q9H0S4|DDX47
     1  MAAPEEHDSP TEASQPIVEE EETKTFKDLG VTDVLCEACD QLGWTKPTKI QIEAIPLALQ
    61  GRDIIGLAET GSGKTGAFAL PILNALLETP QRLFALVLTP TRELAFQISE QFEALGSSIG
   121  VQSAVIVGGI DSMSQSLALA KKPHIIIATP GRLIDHLENT KGFNLRALKY LVMDEADRIL
   181  NMDFETEVDK ILKVIPRDRK TFLFSATMTK KVQKLQRAAL KNPVKCAVSS KYQTVEKLQQ
   241  YYIFIPSKFK DTYLVYILNE LAGNSFMIFC STCNNTQRTA LLLRNLGFTA IPLHGQMSQS
   301  KRLGSLNKFK AKARSILLAT DVASRGLDIP HVDVVVNFDI PTHSKDYIHR VGRTARAGRS
   361  GKAITFVTQY DVELFQRIEH LIGKKLPGFP TQDDEVMMLT ERVAEAQRFA RMELREHGEK
   421  KKRSREDAGD NDDTEGAIGV RNKVAGGKMK KRKGR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX47 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

45 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.98

gnomAD pLI

0

gnomAD missense Z

0.39

DepMap mean gene effect

-1.7

DepMap dependency class

pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• extrinsic apoptotic signaling pathway via death domain receptors
• mRNA processing
• RNA splicing
• rRNA processing

Molecular functions

• ATP binding
• ATP hydrolysis activity
• RNA binding
• RNA helicase activity

Cellular components

• membrane
• nucleolus
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• ATP-dependent RNA helicase DEAD-box, conserved site
• Helicase, C-terminal domain-like
• DEAD/DEAH-box helicase domain
• Helicase superfamily 1/2, ATP-binding domain
• RNA helicase, DEAD-box type, Q motif
• P-loop containing nucleoside triphosphate hydrolase
• DEAD box RNA helicase
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain
• DDX47/Rrp3, DEAD-box helicase domain

KeywordsUniProt

• Acetylation
• Apoptosis
• ATP-binding
• Helicase
• Hydrolase
• mRNA processing
• mRNA splicing
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• RNA-binding
• rRNA processing

InteractionsUniProt · HPA

Protein binding partners of DDX47 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AGO1
• AGO2
• GABARAP
• NOL8
• ABT1