LGALS3

Protein identityUniProt · HPA

UniProt accession

P17931

Gene

LGALS3

Ensembl

ENSG00000131981

Chromosome

14

Canonical length

250 aa

Protein class

Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Predicted secreted proteins

Subcellular location

Nucleoplasm,Plasma membrane,Cytosol

Secretome location

Secreted to blood

OverviewNCBI Gene

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Canonical amino-acid sequenceUniProt

250 residues, UniProt reviewed canonical sequence.

>P17931|LGALS3
     1  MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP
    61  GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL
   121  PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN
   181  WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI
   241  DLTSASYTMI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LGALS3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

1,119 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about LGALS3.

Disease | AutoantibodyPubMed

Conditions in which antibodies against LGALS3 are reported. Each links to that disease's full target list.

• Lupus Erythematosus, Systemic 4

Showing 1 of 2 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for LGALS3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.43

gnomAD pLI

0

gnomAD missense Z

0.33

DepMap mean gene effect

-0.01

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• eosinophil chemotaxis
• epithelial cell differentiation
• innate immune response
• macrophage chemotaxis
• monocyte chemotaxis
• mononuclear cell migration
• mRNA processing
• negative regulation of endocytosis
• negative regulation of extrinsic apoptotic signaling pathway
• negative regulation of immunological synapse formation
• negative regulation of NK T cell activation
• negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
• negative regulation of T cell receptor signaling pathway
• neutrophil chemotaxis
• positive chemotaxis
• positive regulation of calcium ion import
• positive regulation of mononuclear cell migration
• positive regulation of protein localization to plasma membrane
• positive regulation of protein-containing complex assembly
• regulation of extrinsic apoptotic signaling pathway via death domain receptors
• regulation of T cell apoptotic process
• regulation of T cell proliferation
• RNA splicing

Molecular functions

• carbohydrate binding
• chemoattractant activity
• disaccharide binding
• IgE binding
• laminin binding
• molecular condensate scaffold activity
• protein phosphatase binding
• protein phosphatase inhibitor activity
• receptor ligand inhibitor activity
• RNA binding

Cellular components

• cell surface
• cytoplasm
• cytosol
• extracellular exosome
• extracellular matrix
• extracellular region
• extracellular space
• ficolin-1-rich granule membrane
• immunological synapse
• membrane
• mitochondrial inner membrane
• nucleoplasm
• nucleus
• plasma membrane
• secretory granule membrane
• spliceosomal complex

Protein domainsUniProt · Pfam · InterPro

• Galectin, carbohydrate recognition domain
• Concanavalin A-like lectin/glucanase domain superfamily
• Galectin-like
• Galactoside-binding lectin

KeywordsUniProt

• Acetylation
• Cytoplasm
• Differentiation
• Disulfide bond
• IgE-binding protein
• Immunity
• Innate immunity
• Lectin
• mRNA processing
• mRNA splicing
• Nucleus
• Phosphoprotein
• Repeat
• Secreted
• Spliceosome

InteractionsUniProt · HPA

Protein binding partners of LGALS3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ALCAM
• CD6
• DMBT1
• LGALS3BP
• LYPD3
• TRIM16
• UACA
• ZFTRAF1
• CSPG4
• ENG
• LGALS1
• SLC12A7
• SLC15A4
• SLC26A2