Seroatlas · Human Serome Atlas

LEF1

Lymphoid enhancer-binding factor 1

Also known as: LEF1_HUMAN, TCF10, TCF1ALPHA, TCF7L3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UJU2
Gene
LEF1
Ensembl
ENSG00000138795
Chromosome
4
Canonical length
399 aa
Protein class
Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm

OverviewNCBI Gene

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Canonical amino-acid sequenceUniProt

399 residues, UniProt reviewed canonical sequence.

>Q9UJU2|LEF1
     1  MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL ADIKSSLVNE
    61  SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG LYNKGPSYSS YSGYIMMPNM
   121  NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA VHPLTPLITY SDEHFSPGSH PSHIPSDVNS
   181  KQGMSRHPPA PDIPTFYPLS PGGVGQITPP LGWQGQPVYP ITGGFRQPYP SSLSVDTSMS
   241  RFSHHMIPGP PGPHTTGIPH PAIVTPQVKQ EHPHTDSDLM HVKPQHEQRK EQEPKRPHIK
   301  KPLNAFMLYM KEMRANVVAE CTLKESAAIN QILGRRWHAL SREEQAKYYE LARKERQLHM
   361  QLYPGWSARD NYGKKKKRKR EKLQESASGT GPRMTAAYI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LEF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.65
Highest tissue expression
325 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 325 nTPM
  • lymph node: 29 nTPM
  • tonsil: 21 nTPM
  • appendix: 13 nTPM
  • adrenal gland: 12 nTPM
  • testis: 12 nTPM

Single-cell type

  • thymocytes: 347 nCPM
  • choroid plexus epithelial cells: 265 nCPM
  • renal collecting duct intercalated cells: 256 nCPM
  • melanocytes: 214 nCPM
  • t-cells: 142 nCPM
  • ependymal cells: 108 nCPM

Immune cell

  • naive CD4 T-cell: 143 nTPM
  • naive CD8 T-cell: 103 nTPM
  • T-reg: 98 nTPM
  • memory CD4 T-cell: 69 nTPM
  • total PBMC: 37 nTPM
  • NK-cell: 33 nTPM

Brain region

  • thalamus: 42 nTPM
  • choroid plexus: 33 nTPM
  • midbrain: 26 nTPM
  • amygdala: 25 nTPM
  • hypothalamus: 23 nTPM
  • pons: 22 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about LEF1.

Disease | AllUniProt

Conditions LEF1 is implicated in, by any mechanism.

  • Ectodermal dysplasia 17 with or without limb malformations (ECTD17) MIM:621224

Disease | GeneticClinVar

7 pathogenic / likely-pathogenic of 77 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Ectodermal dysplasia 17 with or without limb malformations
  • Ectrodactyly
  • Ectrodactyly and ectodermal dysplasia without cleft lip/palate
  • LEF1-related ectodermal dysplasia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.21
gnomAD pLI
1
gnomAD missense Z
1.57
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of LEF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads LEF1 as an antibody target. Whether an autoantibody or antibody against LEF1 could matter depends on whether native LEF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

LEF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label LEF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/LEF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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