Seroatlas · Human Serome Atlas

KLHL22

Kelch-like protein 22

Also known as: FLJ14360, KELCHL, KLH22_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q53GT1
Gene
KLHL22
Ensembl
ENSG00000099910
Chromosome
22
Canonical length
634 aa
Protein class
Disease related genes, Predicted intracellular proteins
Subcellular location
Vesicles,Microtubules,Cytokinetic bridge,Mitotic spindle

OverviewNCBI Gene

Enables 14-3-3 protein binding activity and ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including cellular response to L-leucine; mitotic spindle assembly checkpoint signaling; and negative regulation of metabolic process. Located in several cellular components, including intercellular bridge; lysosome; and microtubule cytoskeleton. Part of Cul3-RING ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

634 residues, UniProt reviewed canonical sequence.

>Q53GT1|KLHL22
     1  MAEEQEFTQL CKLPAQPSHP HCVNNTYRSA QHSQALLRGL LALRDSGILF DVVLVVEGRH
    61  IEAHRILLAA SCDYFRGMFA GGLKEMEQEE VLIHGVSYNA MCQILHFIYT SELELSLSNV
   121  QETLVAACQL QIPEIIHFCC DFLMSWVDEE NILDVYRLAE LFDLSRLTEQ LDTYILKNFV
   181  AFSRTDKYRQ LPLEKVYSLL SSNRLEVSCE TEVYEGALLY HYSLEQVQAD QISLHEPPKL
   241  LETVRFPLME AEVLQRLHDK LDPSPLRDTV ASALMYHRNE SLQPSLQSPQ TELRSDFQCV
   301  VGFGGIHSTP STVLSDQAKY LNPLLGEWKH FTASLAPRMS NQGIAVLNNF VYLIGGDNNV
   361  QGFRAESRCW RYDPRHNRWF QIQSLQQEHA DLSVCVVGRY IYAVAGRDYH NDLNAVERYD
   421  PATNSWAYVA PLKREVYAHA GATLEGKMYI TCGRRGEDYL KETHCYDPGS NTWHTLADGP
   481  VRRAWHGMAT LLNKLYVIGG SNNDAGYRRD VHQVACYSCT SGQWSSVCPL PAGHGEPGIA
   541  VLDNRIYVLG GRSHNRGSRT GYVHIYDVEK DCWEEGPQLD NSISGLAACV LTLPRSLLLE
   601  PPRGTPDRSQ ADPDFASEVM SVSDWEEFDN SSED

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KLHL22 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
34 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 34 nTPM
  • cerebral cortex: 26 nTPM
  • basal ganglia: 24 nTPM
  • skeletal muscle: 22 nTPM
  • parathyroid gland: 22 nTPM
  • cervix: 21 nTPM

Single-cell type

  • choroid plexus epithelial cells: 107 nCPM
  • myonuclei: 99 nCPM
  • gonadotrophs: 84 nCPM
  • adipocytes: 82 nCPM
  • early primary spermatocytes: 72 nCPM
  • distal convoluted tubule cells: 70 nCPM

Immune cell

  • eosinophil: 19 nTPM
  • T-reg: 19 nTPM
  • NK-cell: 17 nTPM
  • myeloid DC: 16 nTPM
  • neutrophil: 11 nTPM
  • memory CD4 T-cell: 10 nTPM

Brain region

  • cerebellum: 57 nTPM
  • basal ganglia: 53 nTPM
  • cerebral cortex: 52 nTPM
  • hypothalamus: 52 nTPM
  • amygdala: 50 nTPM
  • hippocampal formation: 48 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.44
gnomAD pLI
0.42
gnomAD missense Z
2.41
DepMap mean gene effect
-0.17
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of KLHL22 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads KLHL22 as an antibody target. Whether an autoantibody or antibody against KLHL22 could matter depends on whether native KLHL22 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

KLHL22 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label KLHL22 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/KLHL22. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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