HTR4

Protein identityUniProt · HPA

UniProt accession

Q13639

Gene

HTR4

Ensembl

ENSG00000164270

Chromosome

5

Canonical length

388 aa

Protein class

FDA approved drug targets, G-protein coupled receptors, Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]

Canonical amino-acid sequenceUniProt

388 residues, UniProt reviewed canonical sequence.

>Q13639|HTR4
     1  MDKLDANVSS EEGFGSVEKV VLLTFLSTVI LMAILGNLLV MVAVCWDRQL RKIKTNYFIV
    61  SLAFADLLVS VLVMPFGAIE LVQDIWIYGE VFCLVRTSLD VLLTTASIFH LCCISLDRYY
   121  AICCQPLVYR NKMTPLRIAL MLGGCWVIPT FISFLPIMQG WNNIGIIDLI EKRKFNQNSN
   181  STYCVFMVNK PYAITCSVVA FYIPFLLMVL AYYRIYVTAK EHAHQIQMLQ RAGASSESRP
   241  QSADQHSTHR MRTETKAAKT LCIIMGCFCL CWAPFFVTNI VDPFIDYTVP GQVWTAFLWL
   301  GYINSGLNPF LYAFLNKSFR RAFLIILCCD DERYRRPSIL GQTVPCSTTT INGSTHVLRD
   361  AVECGGQWES QCHPPATSPL VAAQPSDT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HTR4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

7

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

8.5 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for HTR4 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.65

gnomAD pLI

0.02

gnomAD missense Z

0.7

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• adenylate cyclase-activating G protein-coupled receptor signaling pathway
• adenylate cyclase-activating serotonin receptor signaling pathway
• adenylate cyclase-inhibiting serotonin receptor signaling pathway
• chemical synaptic transmission
• G protein-coupled receptor signaling pathway
• G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
• maintenance of gastrointestinal epithelium
• mucus secretion
• regulation of appetite
• regulation of postsynapse assembly
• large intestinal transit

Molecular functions

• G protein-coupled serotonin receptor activity
• neurotransmitter receptor activity
• serotonin binding
• serotonin receptor activity

Cellular components

• cytoplasm
• dendrite
• endosome membrane
• glutamatergic synapse
• membrane
• plasma membrane
• postsynapse

Protein domainsUniProt · Pfam · InterPro

• G protein-coupled receptor, rhodopsin-like
• GPCR, rhodopsin-like, 7TM
• 7 transmembrane receptor (rhodopsin family)
• 5-Hydroxytryptamine 4 receptor

KeywordsUniProt

• Cell membrane
• Disulfide bond
• Endosome
• G-protein coupled receptor
• Glycoprotein
• Membrane
• Receptor
• Transducer
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of HTR4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MAGI2
• MPP3
• NHERF1
• NOS1
• PATJ
• SEC23A
• DPYSL2
• LIN7A
• LIN7C
• GRK5
• GPR37
• GPRIN2