Seroatlas · Human Serome Atlas

GIT2

ARF GTPase-activating protein GIT2

Also known as: GIT2_HUMAN, KIAA0148, PKL

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q14161
Gene
GIT2
Ensembl
ENSG00000139436
Chromosome
12
Canonical length
759 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Microtubules

OverviewNCBI Gene

This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]

Canonical amino-acid sequenceUniProt

759 residues, UniProt reviewed canonical sequence.

>Q14161|GIT2
     1  MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL
    61  LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL
   121  PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI
   181  LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD
   241  HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA
   301  TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS
   361  SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL
   421  SDGPVTVQEF MEVKNALVAS EAKIQQLMKV NNNLSDELRI MQKKLQTLQS ENSNLRKQAT
   481  TNVYQVQTGS EYTDTSNHSS LKRRPSARGS RPMSMYETGS GQKPYLPMGE ASRPEESRMR
   541  LQPFPAHIGR SALVTSSSSL PSFPSTLSWS RDESARRASR LEKQNSTPES DYDNTPNDME
   601  PDGMGSSRKG RQRSMVWPGD GLVPDTAEPH VAPSPTLPST EDVIRKTEQI TKNIQELLRA
   661  AQENKHDSYI PCSERIHVAV TEMAALFPKK PKSDMVRTSL RLLTSSAYRL QSECKKTLPG
   721  DPGSPTDVQL VTQQVIQCAY DIAKAAKQLV TITTKENNN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GIT2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
57 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 57 nTPM
  • thymus: 35 nTPM
  • cerebellum: 34 nTPM
  • spleen: 33 nTPM
  • lymph node: 31 nTPM
  • tonsil: 30 nTPM

Single-cell type

  • neutrophils: 620 nCPM
  • neutrophil progenitors: 382 nCPM
  • monocyte progenitors: 162 nCPM
  • thymocytes: 152 nCPM
  • kupffer cells: 150 nCPM
  • microglia: 149 nCPM

Immune cell

  • eosinophil: 173 nTPM
  • neutrophil: 166 nTPM
  • basophil: 86 nTPM
  • classical monocyte: 76 nTPM
  • myeloid DC: 75 nTPM
  • intermediate monocyte: 72 nTPM

Brain region

  • cerebellum: 42 nTPM
  • basal ganglia: 37 nTPM
  • cerebral cortex: 35 nTPM
  • pons: 34 nTPM
  • hippocampal formation: 34 nTPM
  • white matter: 33 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.27
gnomAD pLI
1
gnomAD missense Z
2.34
DepMap mean gene effect
-0.08
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of GIT2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GIT2 as an antibody target. Whether an autoantibody or antibody against GIT2 could matter depends on whether native GIT2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GIT2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GIT2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/GIT2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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