DDX28

Protein identityUniProt · HPA

UniProt accession

Q9NUL7

Gene

DDX28

Ensembl

ENSG00000182810

Chromosome

16

Canonical length

540 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nucleoli,Mitochondria,Cytosol

OverviewNCBI Gene

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

540 residues, UniProt reviewed canonical sequence.

>Q9NUL7|DDX28
     1  MALTRPVRLF SLVTRLLLAP RRGLTVRSPD EPLPVVRIPV ALQRQLEQRQ SRRRNLPRPV
    61  LVRPGPLLVS ARRPELNQPA RLTLGRWERA PLASQGWKSR RARRDHFSIE RAQQEAPAVR
   121  KLSSKGSFAD LGLEPRVLHA LQEAAPEVVQ PTTVQSSTIP SLLRGRHVVC AAETGSGKTL
   181  SYLLPLLQRL LGQPSLDSLP IPAPRGLVLV PSRELAQQVR AVAQPLGRSL GLLVRDLEGG
   241  HGMRRIRLQL SRQPSADVLV ATPGALWKAL KSRLISLEQL SFLVLDEADT LLDESFLELV
   301  DYILEKSHIA EGPADLEDPF NPKAQLVLVG ATFPEGVGQL LNKVASPDAV TTITSSKLHC
   361  IMPHVKQTFL RLKGADKVAE LVHILKHRDR AERTGPSGTV LVFCNSSSTV NWLGYILDDH
   421  KIQHLRLQGQ MPALMRVGIF QSFQKSSRDI LLCTDIASRG LDSTGVELVV NYDFPPTLQD
   481  YIHRAGRVGR VGSEVPGTVI SFVTHPWDVS LVQKIELAAR RRRSLPGLAS SVKEPLPQAT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

16 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.41

gnomAD pLI

0

gnomAD missense Z

-0.75

DepMap mean gene effect

-0.34

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mitochondrial large ribosomal subunit assembly

Molecular functions

• ATP binding
• ATP hydrolysis activity
• RNA binding
• RNA helicase activity
• rRNA binding

Cellular components

• cytosol
• mitochondrial nucleoid
• mitochondrion
• nucleolus
• nucleoplasm
• ribonucleoprotein granule

Protein domainsUniProt · Pfam · InterPro

• Helicase, C-terminal domain-like
• DEAD/DEAH-box helicase domain
• Helicase superfamily 1/2, ATP-binding domain
• RNA helicase, DEAD-box type, Q motif
• P-loop containing nucleoside triphosphate hydrolase
• DEAD box RNA helicase
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain

KeywordsUniProt

• ATP-binding
• Helicase
• Hydrolase
• Mitochondrion
• Mitochondrion nucleoid
• Nucleotide-binding
• Nucleus
• Ribosome biogenesis
• RNA-binding
• rRNA-binding

InteractionsUniProt · HPA

Protein binding partners of DDX28 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MRM3
• MRPL12
• MRPL2
• RBMS2
• RPUSD4
• UBR5