Seroatlas · Human Serome Atlas

RPUSD4

Pseudouridylate synthase RPUSD4, mitochondrial

Also known as: FLJ14494, RUSD4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96CM3
Gene
RPUSD4
Ensembl
ENSG00000165526
Chromosome
11
Canonical length
377 aa
Protein class
Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Mitochondria

OverviewNCBI Gene

Enables mitochondrial ribosomal large subunit rRNA binding activity; pseudouridine synthase activity; and tRNA binding activity. Involved in positive regulation of mitochondrial translation and pseudouridine synthesis. Located in mitochondrial matrix; nucleoplasm; and ribonucleoprotein granule. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

377 residues, UniProt reviewed canonical sequence.

>Q96CM3|RPUSD4
     1  MAAPRWSASG PWIRGNGQGC GSLFTLVSKP FCAAAAASTA INAQRLAEKL RAQKREQDTK
    61  KEPVSTNAVQ RRVQEIVRFT RQLQRVHPNV LAKALTRGIL HQDKNLVVIN KPYGLPVHGG
   121  PGVQLCITDV LPILAKMLHG HKAEPLHLCH RLDKETTGVM VLAWDKDMAH QVQELFRTRQ
   181  VVKKYWAITV HVPMPSAGVV DIPIVEKEAQ GQQQHHKMTL SPSYRMDDGK MVKVRRSRNA
   241  QVAVTQYQVL SSTLSSALVE LQPITGIKHQ LRVHLSFGLD CPILGDHKYS DWNRLAPQKL
   301  SVGTLKKLGL EQSKARYIPL HLHARQLILP ALGSGKEELN LVCKLPRFFV HSLHRLRLEM
   361  PNEDQNENNE AKCLGAQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPUSD4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
127 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 127 nTPM
  • tongue: 70 nTPM
  • pancreas: 26 nTPM
  • heart muscle: 26 nTPM
  • liver: 20 nTPM
  • stomach: 19 nTPM

Single-cell type

  • myonuclei: 56 nCPM
  • esophageal basal cells: 35 nCPM
  • basal keratinocytes: 28 nCPM
  • paneth cells: 27 nCPM
  • parietal cells: 27 nCPM
  • differentiating spermatogonia: 26 nCPM

Immune cell

  • naive CD4 T-cell: 21 nTPM
  • MAIT T-cell: 20 nTPM
  • T-reg: 18 nTPM
  • memory CD4 T-cell: 18 nTPM
  • naive CD8 T-cell: 17 nTPM
  • myeloid DC: 16 nTPM

Brain region

  • choroid plexus: 11 nTPM
  • spinal cord: 11 nTPM
  • hypothalamus: 10 nTPM
  • pons: 10 nTPM
  • cerebellum: 10 nTPM
  • basal ganglia: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.16
gnomAD pLI
0
gnomAD missense Z
-0.4
DepMap mean gene effect
-0.34
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPUSD4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPUSD4 as an antibody target. Whether an autoantibody or antibody against RPUSD4 could matter depends on whether native RPUSD4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPUSD4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPUSD4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPUSD4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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