MRM3

Protein identityUniProt · HPA

UniProt accession

Q9HC36

Gene

MRM3

Ensembl

ENSG00000171861

Chromosome

17

Canonical length

420 aa

Protein class

Predicted intracellular proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

Canonical amino-acid sequenceUniProt

420 residues, UniProt reviewed canonical sequence.

>Q9HC36|MRM3
     1  MAALVRPARF VVRPLLQVVQ AWDLDARRWV RALRRSPVKV VFPSGEVVEQ KRAPGKQPRK
    61  APSEASAQEQ REKQPLEESA SRAPSTWEES GLRYDKAYPG DRRLSSVMTI VKSRPFREKQ
   121  GKILLEGRRL ISDALKAGAV PKMFFFSRLE YLKELPVDKL KGVSLIKVKF EDIKDWSDLV
   181  TPQGIMGIFA KPDHVKMTYP KTQLQHSLPL LLICDNLRDP GNLGTILRSA AGAGCSKVLL
   241  TKGCVDAWEP KVLRAGMGAH FRMPIINNLE WETVPNYLPP DTRVYVADNC GLYAQAEMSN
   301  KASDHGWVCD QRVMKFHKYE EEEDVETGAS QDWLPHVEVQ SYDSDWTEAP AAVVIGGETY
   361  GVSLESLQLA ESTGGKRLLI PVVPGVDSLN SAMAASILLF EGKRQLRGRA EDLSRDRSYH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRM3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

30 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.42

gnomAD pLI

0

DepMap mean gene effect

-0.13

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mitochondrial large ribosomal subunit assembly
• rRNA 2'-O-methylation
• rRNA processing

Molecular functions

• identical protein binding
• RNA binding
• rRNA (guanosine-2'-O-)-methyltransferase activity

Cellular components

• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• tRNA/rRNA methyltransferase, SpoU type
• RNA 2-O ribose methyltransferase, substrate binding
• tRNA (guanine-N1-)-methyltransferase, N-terminal
• Alpha/beta knot methyltransferases
• Ribosomal protein eL30-like superfamily
• SpoU rRNA Methylase family
• Ribosomal RNA Methyltransferase
• MRM3-like, substrate binding domain
• MRM3-like substrate binding domain

KeywordsUniProt

• Methyltransferase
• Mitochondrion
• rRNA processing
• S-adenosyl-L-methionine
• Transferase
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of MRM3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DDX28
• MRPS30