Seroatlas · Human Serome Atlas

DCAF1

DDB1- and CUL4-associated factor 1

Also known as: DCAF1_HUMAN, KIAA0800, MGC102804, VPRBP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y4B6
Gene: DCAF1
Ensembl: ENSG00000145041
Chromosome: 3
Canonical length: 1507 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli fibrillar center

OverviewNCBI Gene

Enables histone H2AT120 kinase activity; nuclear estrogen receptor binding activity; and ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including cell competition in a multicellular organism; proteasome-mediated ubiquitin-dependent protein catabolic process; and regulation of primary metabolic process. Located in COP9 signalosome; fibrillar center; and nucleoplasm. Part of Cul4-RING E3 ubiquitin ligase complex. Is active in centrosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1507 residues, UniProt reviewed canonical sequence.

>Q9Y4B6|DCAF1
     1  MTTVVVHVDS KAELTTLLEQ WEKEHGSGQD MVPILTRMSQ LIEKETEEYR KGDPDPFDDR
    61  HPGRADPECM LGHLLRILFK NDDFMNALVN AYVMTSREPP LNTAACRLLL DIMPGLETAV
   121  VFQEKEGIVE NLFKWAREAD QPLRTYSTGL LGGAMENQDI AANYRDENSQ LVAIVLRRLR
   181  ELQLQEVALR QENKRPSPRK LSSEPLLPLD EEAVDMDYGD MAVDVVDGDQ EEASGDMEIS
   241  FHLDSGHKTS SRVNSTTKPE DGGLKKNKSA KQGDRENFRK AKQKLGFSSS DPDRMFVELS
   301  NSSWSEMSPW VIGTNYTLYP MTPAIEQRLI LQYLTPLGEY QELLPIFMQL GSRELMMFYI
   361  DLKQTNDVLL TFEALKHLAS LLLHNKFATE FVAHGGVQKL LEIPRPSMAA TGVSMCLYYL
   421  SYNQDAMERV CMHPHNVLSD VVNYTLWLME CSHASGCCHA TMFFSICFSF RAVLELFDRY
   481  DGLRRLVNLI STLEILNLED QGALLSDDEI FASRQTGKHT CMALRKYFEA HLAIKLEQVK
   541  QSLQRTEGGI LVHPQPPYKA CSYTHEQIVE MMEFLIEYGP AQLYWEPAEV FLKLSCVQLL
   601  LQLISIACNW KTYYARNDTV RFALDVLAIL TVVPKIQLQL AESVDVLDEA GSTVSTVGIS
   661  IILGVAEGEF FIHDAEIQKS ALQIIINCVC GPDNRISSIG KFISGTPRRK LPQNPKSSEH
   721  TLAKMWNVVQ SNNGIKVLLS LLSIKMPITD ADQIRALACK ALVGLSRSST VRQIISKLPL
   781  FSSCQIQQLM KEPVLQDKRS DHVKFCKYAA ELIERVSGKP LLIGTDVSLA RLQKADVVAQ
   841  SRISFPEKEL LLLIRNHLIS KGLGETATVL TKEADLPMTA ASHSSAFTPV TAAASPVSLP
   901  RTPRIANGIA TRLGSHAAVG ASAPSAPTAH PQPRPPQGPL ALPGPSYAGN SPLIGRISFI
   961  RERPSPCNGR KIRVLRQKSD HGAYSQSPAI KKQLDRHLPS PPTLDSIITE YLREQHARCK
  1021  NPVATCPPFS LFTPHQCPEP KQRRQAPINF TSRLNRRASF PKYGGVDGGC FDRHLIFSRF
  1081  RPISVFREAN EDESGFTCCA FSARERFLML GTCTGQLKLY NVFSGQEEAS YNCHNSAITH
  1141  LEPSRDGSLL LTSATWSQPL SALWGMKSVF DMKHSFTEDH YVEFSKHSQD RVIGTKGDIA
  1201  HIYDIQTGNK LLTLFNPDLA NNYKRNCATF NPTDDLVLND GVLWDVRSAQ AIHKFDKFNM
  1261  NISGVFHPNG LEVIINTEIW DLRTFHLLHT VPALDQCRVV FNHTGTVMYG AMLQADDEDD
  1321  LMEERMKSPF GSSFRTFNAT DYKPIATIDV KRNIFDLCTD TKDCYLAVIE NQGSMDALNM
  1381  DTVCRLYEVG RQRLAEDEDE EEDQEEEEQE EEDDDEDDDD TDDLDELDTD QLLEAELEED
  1441  DNNENAGEDG DNDFSPSDEE LANLLEEGED GEDEDSDADE EVELILGDTD SSDNSDLEDD
  1501  IILSLNE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DCAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 48 nTPM

Expression across tissuesHPA

Tissue

testis: 48 nTPM
retina: 17 nTPM
skeletal muscle: 16 nTPM
parathyroid gland: 16 nTPM
thymus: 15 nTPM
bone marrow: 14 nTPM

Single-cell type

late spermatids: 540 nCPM
early spermatids: 174 nCPM
myonuclei: 86 nCPM
erythrocyte progenitors: 85 nCPM
cone photoreceptor cells: 81 nCPM
megakaryocyte-erythroid progenitors: 76 nCPM

Immune cell

MAIT T-cell: 6.7 nTPM
T-reg: 6.1 nTPM
gdT-cell: 5.7 nTPM
naive CD4 T-cell: 5.4 nTPM
NK-cell: 5.2 nTPM
memory CD8 T-cell: 5 nTPM

Brain region

cerebellum: 18 nTPM
choroid plexus: 17 nTPM
hypothalamus: 15 nTPM
midbrain: 14 nTPM
white matter: 14 nTPM
basal ganglia: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.11
gnomAD pLI: 1

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

B cell differentiation
negative regulation of transcription by RNA polymerase II
positive regulation of protein catabolic process
post-translational protein modification
proteasome-mediated ubiquitin-dependent protein catabolic process
protein ubiquitination
V(D)J recombination
cell competition in a multicellular organism

Molecular functions

ATP binding
nuclear estrogen receptor binding
protein serine kinase activity
ubiquitin-like ligase-substrate adaptor activity
histone H2AT120 kinase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DCAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DCAF1 as an antibody target. Whether an autoantibody or antibody against DCAF1 could matter depends on whether native DCAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DCAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DCAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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