Seroatlas · Human Serome Atlas

SPAG5

Sperm-associated antigen 5

Also known as: DEEPEST, hMAP126, MAP126, SPAG5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96R06
Gene: SPAG5
Ensembl: ENSG00000076382
Chromosome: 17
Canonical length: 1193 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nuclear bodies,Mitotic spindle,Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1193 residues, UniProt reviewed canonical sequence.

>Q96R06|SPAG5
     1  MWRVKKLSLS LSPSPQTGKP SMRTPLRELT LQPGALTNSG KRSPACSSLT PSLCKLGLQE
    61  GSNNSSPVDF VNNKRTDLSS EHFSHSSKWL ETCQHESDEQ PLDPIPQISS TPKTSEEAVD
   121  PLGNYMVKTI VLVPSPLGQQ QDMIFEARLD TMAETNSISL NGPLRTDDLV REEVAPCMGD
   181  RFSEVAAVSE KPIFQESPSH LLEESPPNPC SEQLHCSKES LSSRTEAVRE DLVPSESNAF
   241  LPSSVLWLSP STALAADFRV NHVDPEEEIV EHGAMEEREM RFPTHPKESE TEDQALVSSV
   301  EDILSTCLTP NLVEMESQEA PGPAVEDVGR ILGSDTESWM SPLAWLEKGV NTSVMLENLR
   361  QSLSLPSMLR DAAIGTTPFS TCSVGTWFTP SAPQEKSTNT SQTGLVGTKH STSETEQLLC
   421  GRPPDLTALS RHDLEDNLLS SLVILEVLSR QLRDWKSQLA VPHPETQDSS TQTDTSHSGI
   481  TNKLQHLKES HEMGQALQQA RNVMQSWVLI SKELISLLHL SLLHLEEDKT TVSQESRRAE
   541  TLVCCCFDLL KKLRAKLQSL KAEREEARHR EEMALRGKDA AEIVLEAFCA HASQRISQLE
   601  QDLASMREFR GLLKDAQTQL VGLHAKQEEL VQQTVSLTST LQQDWRSMQL DYTTWTALLS
   661  RSRQLTEKLT VKSQQALQER DVAIEEKQEV SRVLEQVSAQ LEECKGQTEQ LELENSRLAT
   721  DLRAQLQILA NMDSQLKELQ SQHTHCAQDL AMKDELLCQL TQSNEEQAAQ WQKEEMALKH
   781  MQAELQQQQA VLAKEVRDLK ETLEFADQEN QVAHLELGQV ECQLKTTLEV LRERSLQCEN
   841  LKDTVENLTA KLASTIADNQ EQDLEKTRQY SQKLGLLTEQ LQSLTLFLQT KLKEKTEQET
   901  LLLSTACPPT QEHPLPNDRT FLGSILTAVA DEEPESTPVP LLGSDKSAFT RVASMVSLQP
   961  AETPGMEESL AEMSIMTTEL QSLCSLLQES KEEAIRTLQR KICELQARLQ AQEEQHQEVQ
  1021  KAKEADIEKL NQALCLRYKN EKELQEVIQQ QNEKILEQID KSGELISLRE EVTHLTRSLR
  1081  RAETETKVLQ EALAGQLDSN CQPMATNWIQ EKVWLSQEVD KLRVMFLEMK NEKEKLMIKF
  1141  QSHRNILEEN LRRSDKELEK LDDIVQHIYK TLLSIPEVVR GCKELQGLLE FLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPAG5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.59
Highest tissue expression: 72 nTPM

Expression across tissuesHPA

Tissue

testis: 72 nTPM
kidney: 38 nTPM
bone marrow: 36 nTPM
thymus: 21 nTPM
tonsil: 16 nTPM
liver: 15 nTPM

Single-cell type

late primary spermatocytes: 164 nCPM
early spermatids: 121 nCPM
early primary spermatocytes: 32 nCPM
late spermatids: 29 nCPM
monocyte progenitors: 28 nCPM
erythrocyte progenitors: 21 nCPM

Immune cell

basophil: 12 nTPM
T-reg: 5 nTPM
naive B-cell: 1.4 nTPM
memory CD4 T-cell: 1.2 nTPM
naive CD4 T-cell: 1.1 nTPM
naive CD8 T-cell: 1 nTPM

Brain region

cerebral cortex: 16 nTPM
hypothalamus: 13 nTPM
basal ganglia: 13 nTPM
hippocampal formation: 13 nTPM
white matter: 12 nTPM
midbrain: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.72
gnomAD pLI: 0
gnomAD missense Z: 1.18
DepMap mean gene effect: -0.57
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

microtubule binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Sperm-associated antigen 5

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPAG5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPAG5 as an antibody target. Whether an autoantibody or antibody against SPAG5 could matter depends on whether native SPAG5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPAG5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPAG5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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