SHPRH

Protein identityUniProt · HPA

UniProt accession

Q149N8

Gene

SHPRH

Ensembl

ENSG00000146414

Chromosome

6

Canonical length

1683 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear membrane,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

1683 residues, UniProt reviewed canonical sequence.

>Q149N8|SHPRH
     1  MSSRRKRAPP VRVDEEKRQQ LHWNMHEDRR NEPIIISDDD EQPCPGSDTS SAHYIILSDS
    61  LKEEVAHRDK KRCSKVVSFS KPIEKEETVG IFSPLSVKLN IVISPYHFDN SWKAFLGELT
   121  LQLLPAQSLI ENFSERSITL MSSESSNQFL IYVHSKGEDV EKQKKEPMSI CDKGILVESS
   181  FSGEMLEDLG WLQKKRRIKL YQKPEGNHII KVGIYLLEAG LAKLDFLSDA NSRMKKFNQL
   241  MKKVMEKLHN SIIPDVLEED EDDPESEPEG QDIDELYHFV KQTHQQETQS IQVDVQHPAL
   301  IPVLRPYQRE AVNWMLQQEC FRSSPATESA LHFLWREIVT SEGLKLYYNP YTGCIIREYP
   361  NSGPQLLGGI LADEMGLGKT VEVLALILTH TRQDVKQDAL TLPEGKVVNY FIPSHYFGGK
   421  LKKTEIQNIE FEPKEKVQCP PTRVMILTAV KEMNGKKGVS ILSIYKYVSS IYRYDVQRNR
   481  SLLKRMLKCL IFEGLVKQIK GHGFSGTFTL GKNYKEEDIC DKTKKQAVGS PRKIQKETRK
   541  SGNKDTDSEY LPSDTSDDDD DPYYYYYKSR RNRSKLRKKL VPSTKKGKSQ PFINPDSQGH
   601  CPATSDSGIT DVAMSKSTCI SEFNQEHETE DCAESLNHAD SDVPPSNTMS PFNTSDYRFE
   661  CICGELDQID RKPRVQCLKC HLWQHAKCVN YDEKNLKIKP FYCPHCLVAM EPVSTRATLI
   721  ISPSSICHQW VDEINRHVRS SSLRVLVYQG VKKDGFLQPH FLAEQDIVII TYDVLRSELN
   781  YVDIPHSNSE DGRRLRNQKR YMAIPSPLVA VEWWRICLDE AQMVECPTVK AAEMAQRLSG
   841  INRWCISGTP VQRGLEDLFG LVVFLGIEPY CVKHWWVRLL YRPYCKKNPQ HLYSFIAKIL
   901  WRSAKKDVID QIQIPPQTEE IHWLHFSPVE RHFYHRQHEV CCQDVVVKLR KISDWALKLS
   961  SLDRRTVTSI LYPLLRLRQA CCHPQAVRGE FLPLQKSTMT MEELLTSLQK KCGTECEEAH
  1021  RQLVCALNGL AGIHIIKGEY ALAAELYREV LRSSEEHKGK LKTDSLQRLH ATHNLMELLI
  1081  ARHPGIPPTL RDGRLEEEAK QLREHYMSKC NTEVAEAQQA LYPVQQTIHE LQRKIHSNSP
  1141  WWLNVIHRAI EFTIDEELVQ RVRNEITSNY KQQTGKLSMS EKFRDCRGLQ FLLTTQMEEL
  1201  NKCQKLVREA VKNLEGPPSR NVIESATVCH LRPARLPLNC CVFCKADELF TEYESKLFSN
  1261  TVKGQTAIFE EMIEDEEGLV DDRAPTTTRG LWAISETERS MKAILSFAKS HRFDVEFVDE
  1321  GSTSMDLFEA WKKEYKLLHE YWMALRNRVS AVDELAMATE RLRVRDPREP KPNPPVLHII
  1381  EPHEVEQNRI KLLNDKAVAT SQLQKKLGQL LYLTNLEKSQ DKTSGGVNPE PCPICARQLG
  1441  KQWAVLTCGH CFCNECISII IEQYSVGSHR SSIKCAICRQ TTSHKEISYV FTSEKANQEE
  1501  DIPVKGSHST KVEAVVRTLM KIQLRDPGAK ALVFSTWQDV LDIISKALTD NNMEFAQISR
  1561  VKTFQENLSA FKRDPQINIL LLPLHTGSNG LTIIEATHVL LVEPILNPAH ELQAIGRVHR
  1621  IGQTKPTIVH RFLIKATIEE RMQAMLKTAE RSHTNSSAKH SEASVLTVAD LADLFTKETE
  1681  ELE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SHPRH can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.35

Highest tissue expression

15 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.42

gnomAD pLI

0

gnomAD missense Z

2.33

DepMap mean gene effect

0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA damage response
• DNA repair
• nucleosome assembly
• protein polyubiquitination
• protein ubiquitination

Molecular functions

• ATP binding
• DNA binding
• helicase activity
• hydrolase activity
• ubiquitin protein ligase activity
• ubiquitin protein ligase binding
• ubiquitin-protein transferase activity
• zinc ion binding

Cellular components

• nucleoplasm
• nucleosome

Protein domainsUniProt · Pfam · InterPro

• SNF2, N-terminal domain
• Helicase, C-terminal domain-like
• Zinc finger, RING-type
• Zinc finger, PHD-type
• Linker histone H1/H5, domain H15
• Zinc finger, FYVE/PHD-type
• Zinc finger, RING/FYVE/PHD-type
• Helicase superfamily 1/2, ATP-binding domain
• Zinc finger, RING-type, conserved site
• Zinc finger, PHD-type, conserved site
• Zinc finger, RING-type, eukaryotic
• P-loop containing nucleoside triphosphate hydrolase
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• SNF2-like, N-terminal domain superfamily
• SNF2/RAD5-like, C-terminal helicase domain
• SNF2-related domain
• Helicase conserved C-terminal domain
• linker histone H1 and H5 family
• RING-type zinc-finger
• E3 ubiquitin-protein ligase SHPRH, first helical domain
• E3 ubiquitin-protein ligase SHPRH, second helical domain
• E3 ubiquitin-protein ligase SHPRH-like
• E3 ubiquitin-protein ligase SHPRH, second helical domain
• E3 ubiquitin-protein ligase SHPRH, first helical domain

KeywordsUniProt

• ATP-binding
• DNA damage
• DNA repair
• Helicase
• Hydrolase
• Metal-binding
• Nucleotide-binding
• Phosphoprotein
• Transferase
• Ubl conjugation pathway
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of SHPRH in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HLTF
• PCNA
• RAD18
• UBE2N
• RBBP4