Seroatlas · Human Serome Atlas

RAD54L

DNA repair and recombination protein RAD54-like

Also known as: hHR54, hRAD54, RAD54_HUMAN, RAD54A

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q92698
Gene
RAD54L
Ensembl
ENSG00000085999
Chromosome
1
Canonical length
747 aa
Protein class
Enzymes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm
Quaternary structure
Homohexamer

OverviewNCBI Gene

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008]

Canonical amino-acid sequenceUniProt

747 residues, UniProt reviewed canonical sequence.

>Q92698|RAD54L
     1  MRRSLAPSQL AKRKPEGRSC DDEDWQPGLV TPRKRKSSSE TQIQECFLSP FRKPLSQLTN
    61  QPPCLDSSQH EAFIRSILSK PFKVPIPNYQ GPLGSRALGL KRAGVRRALH DPLEKDALVL
   121  YEPPPLSAHD QLKLDKEKLP VHVVVDPILS KVLRPHQREG VKFLWECVTS RRIPGSHGCI
   181  MADEMGLGKT LQCITLMWTL LRQSPECKPE IDKAVVVSPS SLVKNWYNEV GKWLGGRIQP
   241  LAIDGGSKDE IDQKLEGFMN QRGARVSSPI LIISYETFRL HVGVLQKGSV GLVICDEGHR
   301  LKNSENQTYQ ALDSLNTSRR VLISGTPIQN DLLEYFSLVH FVNSGILGTA HEFKKHFELP
   361  ILKGRDAAAS EADRQLGEER LRELTSIVNR CLIRRTSDIL SKYLPVKIEQ VVCCRLTPLQ
   421  TELYKRFLRQ AKPAEELLEG KMSVSSLSSI TSLKKLCNHP ALIYDKCVEE EDGFVGALDL
   481  FPPGYSSKAL EPQLSGKMLV LDYILAVTRS RSSDKVVLVS NYTQTLDLFE KLCRARRYLY
   541  VRLDGTMSIK KRAKVVERFN SPSSPDFVFM LSSKAGGCGL NLIGANRLVM FDPDWNPAND
   601  EQAMARVWRD GQKKTCYIYR LLSAGTIEEK IFQRQSHKKA LSSCVVDEEQ DVERHFSLGE
   661  LKELFILDEA SLSDTHDRLH CRRCVNSRQI RPPPDGSDCT SDLAGWNHCT DKWGLRDEVL
   721  QAAWDAASTA ITFVFHQRSH EEQRGLR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAD54L can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
14 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 14 nTPM
  • testis: 12 nTPM
  • bone marrow: 8.2 nTPM
  • tonsil: 7.8 nTPM
  • lymph node: 5.1 nTPM
  • esophagus: 4.8 nTPM

Single-cell type

  • endometrial luminal cells: 489 nCPM
  • endometrial glandular cells: 190 nCPM
  • epicardial cells: 73 nCPM
  • endometrial secretory cells: 49 nCPM
  • endometrial ciliated cells: 41 nCPM
  • monocyte progenitors: 26 nCPM

Immune cell

  • T-reg: 1.1 nTPM
  • NK-cell: 0.3 nTPM
  • gdT-cell: 0.2 nTPM
  • total PBMC: 0.2 nTPM
  • memory CD8 T-cell: 0.1 nTPM
  • basophil: 0 nTPM

Brain region

  • cerebellum: 5.6 nTPM
  • cerebral cortex: 4.8 nTPM
  • basal ganglia: 4.5 nTPM
  • pons: 4.5 nTPM
  • white matter: 4.4 nTPM
  • medulla oblongata: 3.5 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RAD54L.

Disease | GeneticClinVar

9 pathogenic / likely-pathogenic of 1,109 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.97
gnomAD pLI
0
gnomAD missense Z
-0.25
DepMap mean gene effect
-0.2
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RAD54L in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RAD54L as an antibody target. Whether an autoantibody or antibody against RAD54L could matter depends on whether native RAD54L is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RAD54L is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RAD54L as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RAD54L. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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