Seroatlas · Human Serome Atlas

PSMF1

Proteasome inhibitor PI31 subunit

Also known as: PI31, PSMF1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q92530
Gene: PSMF1
Ensembl: ENSG00000125818
Chromosome: 20
Canonical length: 271 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

271 residues, UniProt reviewed canonical sequence.

>Q92530|PSMF1
     1  MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYFGLGV GDQPGPNDKK SELLPAGWNN
    61  NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ VADLTLNLDD YIDAEHLGDF
   121  HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN VSSPHREFPP ATAREVDPLR IPPHHPHTSR
   181  QPPWCDPLGP FVVGGEDLDP FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG
   241  ARFDPFGPIG TSPPGPNPDH LPPPGYDDMY L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PSMF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 121 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 121 nTPM
testis: 95 nTPM
tongue: 78 nTPM
liver: 68 nTPM
blood vessel: 55 nTPM
pancreas: 54 nTPM

Single-cell type

late spermatids: 6,955 nCPM
early spermatids: 1,485 nCPM
late primary spermatocytes: 412 nCPM
erythrocyte progenitors: 170 nCPM
esophageal suprabasal cells: 148 nCPM
esophageal apical cells: 144 nCPM

Immune cell

basophil: 125 nTPM
eosinophil: 124 nTPM
non-classical monocyte: 84 nTPM
T-reg: 82 nTPM
intermediate monocyte: 81 nTPM
memory B-cell: 74 nTPM

Brain region

thalamus: 57 nTPM
medulla oblongata: 55 nTPM
white matter: 53 nTPM
pons: 52 nTPM
cerebral cortex: 52 nTPM
midbrain: 51 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.5
gnomAD pLI: 0
gnomAD missense Z: -0.06
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 14% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

PI31 proteasome regulator, N-terminal
PI31 proteasome regulator N-terminal
PI31 proteasome regulator, C-terminal
Proteasome inhibitor PI31-like
PI31 proteasome regulator

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PSMF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PSMF1 as an antibody target. Whether an autoantibody or antibody against PSMF1 could matter depends on whether native PSMF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PSMF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PSMF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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