Seroatlas · Human Serome Atlas

PRKAG3

5'-AMP-activated protein kinase subunit gamma-3

Also known as: AAKG3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UGI9
Gene: PRKAG3
Ensembl: ENSG00000115592
Chromosome: 2
Canonical length: 489 aa
Protein class: FDA approved drug targets, Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

489 residues, UniProt reviewed canonical sequence.

>Q9UGI9|PRKAG3
     1  MEPGLEHALR RTPSWSSLGG SEHQEMSFLE QENSSSWPSP AVTSSSERIR GKRRAKALRW
    61  TRQKSVEEGE PPGQGEGPRS RPAAESTGLE ATFPKTTPLA QADPAGVGTP PTGWDCLPSD
   121  CTASAAGSST DDVELATEFP ATEAWECELE GLLEERPALC LSPQAPFPKL GWDDELRKPG
   181  AQIYMRFMQE HTCYDAMATS SKLVIFDTML EIKKAFFALV ANGVRAAPLW DSKKQSFVGM
   241  LTITDFILVL HRYYRSPLVQ IYEIEQHKIE TWREIYLQGC FKPLVSISPN DSLFEAVYTL
   301  IKNRIHRLPV LDPVSGNVLH ILTHKRLLKF LHIFGSLLPR PSFLYRTIQD LGIGTFRDLA
   361  VVLETAPILT ALDIFVDRRV SALPVVNECG QVVGLYSRFD VIHLAAQQTY NHLDMSVGEA
   421  LRQRTLCLEG VLSCQPHESL GEVIDRIARE QVHRLVLVDE TQHLLGVVSL SDILQALVLS
   481  PAGIDALGA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRKAG3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 78 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 78 nTPM
tongue: 16 nTPM
esophagus: 1.1 nTPM
prostate: 0.9 nTPM
salivary gland: 0.7 nTPM
basal ganglia: 0.5 nTPM

Single-cell type

myonuclei: 50 nCPM
thymic myoid cells: 30 nCPM
epicardial cells: 4.5 nCPM
basal prostatic cells: 3 nCPM
epididymal basal cells: 2.3 nCPM
leydig cells: 1.2 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

basal ganglia: 3.1 nTPM
thalamus: 1.1 nTPM
choroid plexus: 0.8 nTPM
white matter: 0.6 nTPM
cerebral cortex: 0.5 nTPM
hippocampal formation: 0.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.19
gnomAD pLI: 0
gnomAD missense Z: -0.27
DepMap mean gene effect: 0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRKAG3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRKAG3 as an antibody target. Whether an autoantibody or antibody against PRKAG3 could matter depends on whether native PRKAG3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRKAG3 is annotated as secreted, so native PRKAG3 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label PRKAG3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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