Seroatlas · Human Serome Atlas

NFYA

Nuclear transcription factor Y subunit alpha

Also known as: CBF-B, HAP2, NF-YA, NFYA_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P23511
Gene: NFYA
Ensembl: ENSG00000001167
Chromosome: 6
Canonical length: 347 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

347 residues, UniProt reviewed canonical sequence.

>P23511|NFYA
     1  MEQYTANSNS STEQIVVQAG QIQQQQQGGV TAVQLQTEAQ VASASGQQVQ TLQVVQGQPL
    61  MVQVSGGQLI TSTGQPIMVQ AVPGGQGQTI MQVPVSGTQG LQQIQLVPPG QIQIQGGQAV
   121  QVQGQQGQTQ QIIIQQPQTA VTAGQTQTQQ QIAVQGQQVA QTAEGQTIVY QPVNADGTIL
   181  QQVTVPVSGM ITIPAASLAG AQIVQTGANT NTTSSGQGTV TVTLPVAGNV VNSGGMVMMV
   241  PGAGSVPAIQ RIPLPGAEML EEEPLYVNAK QYHRILKRRQ ARAKLEAEGK IPKERRKYLH
   301  ESRHRHAMAR KRGEGGRFFS PKEKDSPHMQ DPNQADEEAM TQIIRVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NFYA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.6
Highest tissue expression: 36 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 36 nTPM
thymus: 36 nTPM
testis: 34 nTPM
retina: 18 nTPM
tonsil: 17 nTPM
lymph node: 16 nTPM

Single-cell type

neutrophils: 181 nCPM
neutrophil progenitors: 87 nCPM
late spermatids: 69 nCPM
early spermatids: 63 nCPM
monocyte progenitors: 48 nCPM
late primary spermatocytes: 42 nCPM

Immune cell

neutrophil: 7.5 nTPM
MAIT T-cell: 7.2 nTPM
NK-cell: 6.4 nTPM
T-reg: 6.4 nTPM
naive CD8 T-cell: 6 nTPM
memory CD8 T-cell: 5.9 nTPM

Brain region

white matter: 49 nTPM
basal ganglia: 38 nTPM
midbrain: 35 nTPM
medulla oblongata: 34 nTPM
pons: 33 nTPM
thalamus: 32 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.51
gnomAD pLI: 0.15
gnomAD missense Z: 2.78
DepMap mean gene effect: -0.29
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Nuclear transcription factor Y subunit A
CCAAT-binding factor, conserved site
CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NFYA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NFYA as an antibody target. Whether an autoantibody or antibody against NFYA could matter depends on whether native NFYA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NFYA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NFYA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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