ZHX3

Protein identityUniProt · HPA

UniProt accession

Q9H4I2

Gene

ZHX3

Ensembl

ENSG00000174306

Chromosome

20

Canonical length

956 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the zinc fingers and homeoboxes (ZHX) gene family. The encoded protein contains two C2H2-type zinc fingers and five homeodomains and forms a dimer with itself or with zinc fingers and homeoboxes family member 1. In the nucleus, the dimerized protein interacts with the A subunit of the ubiquitous transcription factor nuclear factor-Y and may function as a transcriptional repressor. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

956 residues, UniProt reviewed canonical sequence.

>Q9H4I2|ZHX3
     1  MASKRKSTTP CMIPVKTVVL QDASMEAQPA ETLPEGPQQD LPPEASAASS EAAQNPSSTD
    61  GSTLANGHRS TLDGYLYSCK YCDFRSHDMT QFVGHMNSEH TDFNKDPTFV CSGCSFLAKT
   121  PEGLSLHNAT CHSGEASFVW NVAKPDNHVV VEQSIPESTS TPDLAGEPSA EGADGQAEII
   181  ITKTPIMKIM KGKAEAKKIH TLKENVPSQP VGEALPKLST GEMEVREGDH SFINGAVPVS
   241  QASASSAKNP HAANGPLIGT VPVLPAGIAQ FLSLQQQPPV HAQHHVHQPL PTAKALPKVM
   301  IPLSSIPTYN AAMDSNSFLK NSFHKFPYPT KAELCYLTVV TKYPEEQLKI WFTAQRLKQG
   361  ISWSPEEIED ARKKMFNTVI QSVPQPTITV LNTPLVASAG NVQHLIQAAL PGHVVGQPEG
   421  TGGGLLVTQP LMANGLQATS SPLPLTVTSV PKQPGVAPIN TVCSNTTSAV KVVNAAQSLL
   481  TACPSITSQA FLDASIYKNK KSHEQLSALK GSFCRNQFPG QSEVEHLTKV TGLSTREVRK
   541  WFSDRRYHCR NLKGSRAMIP GDHSSIIIDS VPEVSFSPSS KVPEVTCIPT TATLATHPSA
   601  KRQSWHQTPD FTPTKYKERA PEQLRALESS FAQNPLPLDE ELDRLRSETK MTRREIDSWF
   661  SERRKKVNAE ETKKAEENAS QEEEEAAEDE GGEEDLASEL RVSGENGSLE MPSSHILAER
   721  KVSPIKINLK NLRVTEANGR NEIPGLGACD PEDDESNKLA EQLPGKVSCK KTAQQRHLLR
   781  QLFVQTQWPS NQDYDSIMAQ TGLPRPEVVR WFGDSRYALK NGQLKWYEDY KRGNFPPGLL
   841  VIAPGNRELL QDYYMTHKML YEEDLQNLCD KTQMSSQQVK QWFAEKMGEE TRAVADTGSE
   901  DQGPGTGELT AVHKGMGDTY SEVSENSESW EPRVPEASSE PFDTSSPQAG RQLETD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZHX3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.56

Highest tissue expression

33 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.5

gnomAD pLI

0.05

gnomAD missense Z

1.46

DepMap mean gene effect

0.07

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell differentiation
• negative regulation of DNA-templated transcription
• negative regulation of transcription by RNA polymerase II
• positive regulation of osteoblast differentiation
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• protein heterodimerization activity
• protein homodimerization activity
• RNA polymerase II transcription regulatory region sequence-specific DNA binding
• zinc ion binding

Cellular components

• chromatin
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Homeodomain
• Homedomain-like superfamily
• Zinc finger C2H2-type
• Homez, homeobox domain
• Zinc finger C2H2 superfamily
• ZHX, C2H2 finger domain
• Homeodomain
• Homeodomain leucine-zipper encoding, Homez
• Zinc-fingers and homeoboxes C2H2 finger domain

KeywordsUniProt

• Differentiation
• DNA-binding
• Homeobox
• Metal-binding
• Nucleus
• Phosphoprotein
• Repeat
• Repressor
• Transcription
• Transcription regulation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of ZHX3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NFYA