NFYB
Nuclear transcription factor Y subunit beta
Also known as: CBF-A, HAP3, NF-YB, NFYB_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P25208
- Gene
- NFYB
- Ensembl
- ENSG00000120837
- Chromosome
- 12
- Canonical length
- 207 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
207 residues, UniProt reviewed canonical sequence.
>P25208|NFYB
1 MTMDGDSSTT DASQLGISAD YIGGSHYVIQ PHDDTEDSMN DHEDTNGSKE SFREQDIYLP
61 IANVARIMKN AIPQTGKIAK DAKECVQECV SEFISFITSE ASERCHQEKR KTINGEDILF
121 AMSTLGFDSY VEPLKLYLQK FREAMKGEKG IGGAVTATDG LSEELTEEAF TNQLPAGLIT
181 TDGQQQNVMV YTTSYQQISG VQQIQFSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NFYB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.55
- Highest tissue expression
- 64 nTPM
Expression across tissuesHPA
Tissue
- seminal vesicle: 64 nTPM
- spinal cord: 50 nTPM
- blood vessel: 50 nTPM
- colon: 46 nTPM
- midbrain: 42 nTPM
- hippocampal formation: 38 nTPM
Single-cell type
- syncytiotrophoblasts: 486 nCPM
- extravillous trophoblasts: 109 nCPM
- early primary spermatocytes: 77 nCPM
- migrating cytotrophoblasts: 73 nCPM
- mesothelial cells: 69 nCPM
- cytotrophoblasts: 67 nCPM
Immune cell
- eosinophil: 19 nTPM
- basophil: 18 nTPM
- T-reg: 17 nTPM
- MAIT T-cell: 17 nTPM
- naive CD8 T-cell: 14 nTPM
- NK-cell: 14 nTPM
Brain region
- white matter: 36 nTPM
- spinal cord: 34 nTPM
- basal ganglia: 32 nTPM
- amygdala: 31 nTPM
- midbrain: 31 nTPM
- medulla oblongata: 29 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.21
- gnomAD pLI
- 0
- gnomAD missense Z
- 2.18
- DepMap mean gene effect
- -0.78
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to leukemia inhibitory factor
- positive regulation of transcription by RNA polymerase II
- regulation of DNA-templated transcription
- regulation of transcription by RNA polymerase II
Molecular functions
- DNA binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription factor binding
- protein heterodimerization activity
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Transcription factor CBF/NF-Y/archaeal histone domain
- Histone-fold
- Histone-like transcription factor (CBF/NF-Y) and archaeal histone
- Transcription factor, NFYB/HAP3, conserved site
- Transcription factor NFYB/HAP3
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NFYB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NFYB as an antibody target. Whether an autoantibody or antibody against NFYB could matter depends on whether native NFYB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NFYB is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NFYB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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