Seroatlas · Human Serome Atlas

NFKBIE

NF-kappa-B inhibitor epsilon

Also known as: IKBE, IKBE_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O00221
Gene
NFKBIE
Ensembl
ENSG00000146232
Chromosome
6
Canonical length
500 aa
Protein class
Cancer-related genes, Human disease related genes, Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm,Nucleoli fibrillar center,Cytosol

OverviewNCBI Gene

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

500 residues, UniProt reviewed canonical sequence.

>O00221|NFKBIE
     1  MNQRRSESRP GNHRLQAYAE PGKGDSGGAG PLSGSARRGR GGGGAIRVRR PCWSGGAGRG
    61  GGPAWAVRLP TVTAGWTWPA LRTLSSLRAG PSEPHSPGRR PPRAGRPLCQ ADPQPGKAAR
   121  RSLEPDPAQT GPRPARAAGM SEARKGPDEA EESQYDSGIE SLRSLRSLPE STSAPASGPS
   181  DGSPQPCTHP PGPVKEPQEK EDADGERADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH
   241  VGALSPQQLE ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL
   301  AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR PEPGRGTSHS
   361  LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE GTSGKTALHL AVETQERGLV
   421  QFLLQAGAQV DARMLNGCTP LHLAAGRGLM GISSTLCKAG ADSLLRNVED ETPQDLTEES
   481  LVLLPFDDLK ISGKLLLCTD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NFKBIE can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.49
Highest tissue expression
34 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 34 nTPM
  • bone marrow: 30 nTPM
  • tonsil: 24 nTPM
  • lymph node: 19 nTPM
  • appendix: 18 nTPM
  • urinary bladder: 15 nTPM

Single-cell type

  • epididymal clear cells: 78 nCPM
  • cdc: 74 nCPM
  • hofbauer cells: 63 nCPM
  • late spermatids: 59 nCPM
  • neutrophils: 53 nCPM
  • pancreatic duct cells: 51 nCPM

Immune cell

  • non-classical monocyte: 11 nTPM
  • intermediate monocyte: 9.5 nTPM
  • basophil: 8.9 nTPM
  • memory B-cell: 8.6 nTPM
  • plasmacytoid DC: 8.1 nTPM
  • classical monocyte: 7.5 nTPM

Brain region

  • cerebral cortex: 9.7 nTPM
  • white matter: 8.6 nTPM
  • cerebellum: 8 nTPM
  • thalamus: 7.9 nTPM
  • spinal cord: 7.4 nTPM
  • medulla oblongata: 7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.43
gnomAD pLI
0.77
gnomAD missense Z
1.47
DepMap mean gene effect
-0.38
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NFKBIE in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NFKBIE as an antibody target. Whether an autoantibody or antibody against NFKBIE could matter depends on whether native NFKBIE is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NFKBIE is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NFKBIE as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NFKBIE. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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