Seroatlas · Human Serome Atlas

MARK4

MAP/microtubule affinity-regulating kinase 4

Also known as: FLJ90097, KIAA1860, MARK4_HUMAN, MARKL1, Nbla00650, PAR-1D

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96L34
Gene
MARK4
Ensembl
ENSG00000007047
Chromosome
19
Canonical length
752 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Canonical amino-acid sequenceUniProt

752 residues, UniProt reviewed canonical sequence.

>Q96L34|MARK4
     1  MSSRTVLAPG NDRNSDTHGT LGSGRSSDKG PSWSSRSLGA RCRNSIASCP EEQPHVGNYR
    61  LLRTIGKGNF AKVKLARHIL TGREVAIKII DKTQLNPSSL QKLFREVRIM KGLNHPNIVK
   121  LFEVIETEKT LYLVMEYASA GEVFDYLVSH GRMKEKEARA KFRQIVSAVH YCHQKNIVHR
   181  DLKAENLLLD AEANIKIADF GFSNEFTLGS KLDTFCGSPP YAAPELFQGK KYDGPEVDIW
   241  SLGVILYTLV SGSLPFDGHN LKELRERVLR GKYRVPFYMS TDCESILRRF LVLNPAKRCT
   301  LEQIMKDKWI NIGYEGEELK PYTEPEEDFG DTKRIEVMVG MGYTREEIKE SLTSQKYNEV
   361  TATYLLLGRK TEEGGDRGAP GLALARVRAP SDTTNGTSSS KGTSHSKGQR SSSSTYHRQR
   421  RHSDFCGPSP APLHPKRSPT STGEAELKEE RLPGRKASCS TAGSGSRGLP PSSPMVSSAH
   481  NPNKAEIPER RKDSTSTPNN LPPSMMTRRN TYVCTERPGA ERPSLLPNGK ENSSGTPRVP
   541  PASPSSHSLA PPSGERSRLA RGSTIRSTFH GGQVRDRRAG GGGGGGVQNG PPASPTLAHE
   601  AAPLPAGRPR PTTNLFTKLT SKLTRRVADE PERIGGPEVT SCHLPWDQTE TAPRLLRFPW
   661  SVKLTSSRPP EALMAALRQA TAAARCRCRQ PQPFLLACLH GGAGGPEPLS HFEVEVCQLP
   721  RPGLRGVLFR RVAGTALAFR TLVTRISNDL EL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
36 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 36 nTPM
  • testis: 26 nTPM
  • cerebral cortex: 22 nTPM
  • basal ganglia: 22 nTPM
  • hippocampal formation: 21 nTPM
  • skin: 19 nTPM

Single-cell type

  • retinal horizontal cells: 371 nCPM
  • sertoli cells: 328 nCPM
  • retinal amacrine cells: 119 nCPM
  • suprabasal keratinocytes: 105 nCPM
  • basal keratinocytes: 102 nCPM
  • cone photoreceptor cells: 97 nCPM

Immune cell

  • memory CD8 T-cell: 0.5 nTPM
  • basophil: 0.4 nTPM
  • classical monocyte: 0.4 nTPM
  • gdT-cell: 0.4 nTPM
  • naive CD8 T-cell: 0.3 nTPM
  • neutrophil: 0.3 nTPM

Brain region

  • cerebral cortex: 52 nTPM
  • basal ganglia: 49 nTPM
  • thalamus: 47 nTPM
  • hippocampal formation: 47 nTPM
  • amygdala: 45 nTPM
  • midbrain: 42 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.26
gnomAD pLI
1
gnomAD missense Z
2.92
DepMap mean gene effect
-0.05
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MARK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARK4 as an antibody target. Whether an autoantibody or antibody against MARK4 could matter depends on whether native MARK4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARK4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MARK4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MARK4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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