Seroatlas · Human Serome Atlas

BRF2

Transcription factor IIIB 50 kDa subunit

Also known as: BRF2_HUMAN, BRFU, FLJ11052, TFIIIB50

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HAW0
Gene: BRF2
Ensembl: ENSG00000104221
Chromosome: 8
Canonical length: 419 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli

OverviewNCBI Gene

This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

419 residues, UniProt reviewed canonical sequence.

>Q9HAW0|BRF2
     1  MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL REVTYSRSTG
    61  ENEQVSRSQQ RGLRRVRDLC RVLQLPPTFE DTAVAYYQQA YRHSGIRAAR LQKKEVLVGC
   121  CVLITCRQHN WPLTMGAICT LLYADLDVFS STYMQIVKLL GLDVPSLCLA ELVKTYCSSF
   181  KLFQASPSVP AKYVEDKEKM LSRTMQLVEL ANETWLVTGR HPLPVITAAT FLAWQSLQPA
   241  DRLSCSLARF CKLANVDLPY PASSRLQELL AVLLRMAEQL AWLRVLRLDK RSVVKHIGDL
   301  LQHRQSLVRS AFRDGTAEVE TREKEPPGWG QGQGEGEVGN NSLGLPQGKR PASPALLLPP
   361  CMLKSPKRIC PVPPVSTVTG DENISDSEIE QYLRTPQEVR DFQRAQAARQ AATSVPNPP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BRF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.38
Highest tissue expression: 27 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 27 nTPM
bone marrow: 14 nTPM
tongue: 12 nTPM
ovary: 11 nTPM
thymus: 10 nTPM
cervix: 9.4 nTPM

Single-cell type

endometrial luminal cells: 51 nCPM
endometrial glandular cells: 30 nCPM
cytotrophoblasts: 28 nCPM
migrating cytotrophoblasts: 19 nCPM
alveolar cells type 1: 17 nCPM
syncytiotrophoblasts: 17 nCPM

Immune cell

naive CD4 T-cell: 20 nTPM
NK-cell: 20 nTPM
naive CD8 T-cell: 17 nTPM
naive B-cell: 15 nTPM
memory CD4 T-cell: 14 nTPM
memory B-cell: 14 nTPM

Brain region

pons: 13 nTPM
hippocampal formation: 13 nTPM
cerebral cortex: 12 nTPM
white matter: 12 nTPM
midbrain: 12 nTPM
basal ganglia: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.2
gnomAD pLI: 0
gnomAD missense Z: -0.02
DepMap mean gene effect: -1.74
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Transcription factor TFIIB
Zinc finger, TFIIB-type
Cyclin-like superfamily
TFIIB zinc-binding
BRF2-like, C-terminal
BRF2-like, C-terminal cyclin repeat

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of BRF2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads BRF2 as an antibody target. Whether an autoantibody or antibody against BRF2 could matter depends on whether native BRF2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

BRF2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label BRF2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...