Seroatlas · Human Serome Atlas

BRF1

Transcription factor IIIB 90 kDa subunit

Also known as: BRF, GTF3B, hBRF, TAF3B2, TAF3C, TF3B_HUMAN, TFIIIB90

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q92994
Gene
BRF1
Ensembl
ENSG00000185024
Chromosome
14
Canonical length
677 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]

Canonical amino-acid sequenceUniProt

677 residues, UniProt reviewed canonical sequence.

>Q92994|BRF1
     1  MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL
    61  DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR
   121  HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA
   181  IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH
   241  DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK
   301  LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GGLASLAKDG STEDTASSLC
   361  GEEDTEDEEL EAAASHLNKD LYRELLGGAP GSSEAAGSPE WGGRPPALGS LLDPLPTAAS
   421  LGISDSIREC ISSQSSDPKD ASGDGELDLS GIDDLEIDRY ILNESEARVK AELWMRENAE
   481  YLREQREKEA RIAKEKELGI YKEHKPKKSC KRREPIQAST AREAIEKMLE QKKISSKINY
   541  SVLRGLSSAG GGSPHREDAQ PEHSASARKL SRRRTPASRS GADPVTSVGK RLRPLVSTQP
   601  AKKVATGEAL LPSSPTLGAE PARPQAVLVE SGPVSYHADE EADEEEPDEE DGEPCVSALQ
   661  MMGSNDYGCD GDEDDGY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BRF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.51
Highest tissue expression
27 nTPM

Expression across tissuesHPA

Tissue

  • spinal cord: 27 nTPM
  • cerebellum: 25 nTPM
  • hypothalamus: 22 nTPM
  • cerebral cortex: 22 nTPM
  • midbrain: 20 nTPM
  • hippocampal formation: 20 nTPM

Single-cell type

  • epicardial cells: 96 nCPM
  • hematopoietic stem cells: 82 nCPM
  • oligodendrocytes: 59 nCPM
  • pdcs: 59 nCPM
  • retinal horizontal cells: 58 nCPM
  • adrenal medulla cells: 53 nCPM

Immune cell

  • non-classical monocyte: 4 nTPM
  • plasmacytoid DC: 3.2 nTPM
  • NK-cell: 2.5 nTPM
  • eosinophil: 1.9 nTPM
  • gdT-cell: 1.6 nTPM
  • memory CD8 T-cell: 1.6 nTPM

Brain region

  • white matter: 79 nTPM
  • medulla oblongata: 73 nTPM
  • pons: 67 nTPM
  • thalamus: 59 nTPM
  • midbrain: 58 nTPM
  • cerebral cortex: 57 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about BRF1.

Disease | AllUniProt

Conditions BRF1 is implicated in, by any mechanism.

Disease | GeneticClinVar

13 pathogenic / likely-pathogenic of 261 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.6
gnomAD pLI
0
gnomAD missense Z
0.14
DepMap mean gene effect
-1.26
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of BRF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads BRF1 as an antibody target. Whether an autoantibody or antibody against BRF1 could matter depends on whether native BRF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

BRF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label BRF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/BRF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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