Seroatlas · Human Serome Atlas

ITSN2

Intersectin-2

Also known as: ITSN2_HUMAN, KIAA1256, PRO2015, SH3D1B, SH3P18, SWA, SWAP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NZM3
Gene: ITSN2
Ensembl: ENSG00000198399
Chromosome: 2
Canonical length: 1697 aa
Protein class: Predicted intracellular proteins
Subcellular location: Vesicles,Plasma membrane

OverviewNCBI Gene

This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017]

Canonical amino-acid sequenceUniProt

1697 residues, UniProt reviewed canonical sequence.

>Q9NZM3|ITSN2
     1  MMAQFPTAMN GGPNMWAITS EERTKHDRQF DNLKPSGGYI TGDQARNFFL QSGLPAPVLA
    61  EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP PIMKQPPMFS PLISARFGMG
   121  SMPNLSIPQP LPPAAPITSL SSATSGTNLP PLMMPTPLVP SVSTSSLPNG TASLIQPLPI
   181  PYSSSTLPHG SSYSLMMGGF GGASIQKAQS LIDLGSSSST SSTASLSGNS PKTGTSEWAV
   241  PQPTRLKYRQ KFNTLDKSMS GYLSGFQARN ALLQSNLSQT QLATIWTLAD VDGDGQLKAE
   301  EFILAMHLTD MAKAGQPLPL TLPPELVPPS FRGGKQIDSI NGTLPSYQKM QEEEPQKKLP
   361  VTFEDKRKAN YERGNMELEK RRQALMEQQQ REAERKAQKE KEEWERKQRE LQEQEWKKQL
   421  ELEKRLEKQR ELERQREEER RKDIERREAA KQELERQRRL EWERIRRQEL LNQKNREQEE
   481  IVRLNSKKKN LHLELEALNG KHQQISGRLQ DVRLKKQTQK TELEVLDKQC DLEIMEIKQL
   541  QQELQEYQNK LIYLVPEKQL LNERIKNMQF SNTPDSGVSL LHKKSLEKEE LCQRLKEQLD
   601  ALEKETASKL SEMDSFNNQL KCGNMDDSVL QCLLSLLSCL NNLFLLLKEL RETYNTQQLA
   661  LEQLYKIKRD KLKEIERKRL ELMQKKKLED EAARKAKQGK ENLWKENLRK EEEEKQKRLQ
   721  EEKTQEKIQE EERKAEEKQR KDKDTLKAEE KKRETASVLV NYRALYPFEA RNHDEMSFNS
   781  GDIIQVDEKT VGEPGWLYGS FQGNFGWFPC NYVEKMPSSE NEKAVSPKKA LLPPTVSLSA
   841  TSTSSEPLSS NQPASVTDYQ NVSFSNLTVN TSWQKKSAFT RTVSPGSVSP IHGQGQVVEN
   901  LKAQALCSWT AKKDNHLNFS KHDIITVLEQ QENWWFGEVH GGRGWFPKSY VKIIPGSEVK
   961  REEPEALYAA VNKKPTSAAY SVGEEYIALY PYSSVEPGDL TFTEGEEILV TQKDGEWWTG
  1021  SIGDRSGIFP SNYVKPKDQE SFGSASKSGA SNKKPEIAQV TSAYVASGSE QLSLAPGQLI
  1081  LILKKNTSGW WQGELQARGK KRQKGWFPAS HVKLLGPSSE RATPAFHPVC QVIAMYDYAA
  1141  NNEDELSFSK GQLINVMNKD DPDWWQGEIN GVTGLFPSNY VKMTTDSDPS QQWCADLQTL
  1201  DTMQPIERKR QGYIHELIQT EERYMADLQL VVEVFQKRMA ESGFLTEGEM ALIFVNWKEL
  1261  IMSNTKLLKA LRVRKKTGGE KMPVQMIGDI LAAELSHMQA YIRFCSCQLN GAALLQQKTD
  1321  EDTDFKEFLK KLASDPRCKG MPLSSFLLKP MQRITRYPLL IRSILENTPE SHADHSSLKL
  1381  ALERAEELCS QVNEGVREKE NSDRLEWIQA HVQCEGLAEQ LIFNSLTNCL GPRKLLHSGK
  1441  LYKTKSNKEL HGFLFNDFLL LTYMVKQFAV SSGSEKLFSS KSNAQFKMYK TPIFLNEVLV
  1501  KLPTDPSSDE PVFHISHIDR VYTLRTDNIN ERTAWVQKIK AASEQYIDTE KKKREKAYQA
  1561  RSQKTSGIGR LMVHVIEATE LKACKPNGKS NPYCEISMGS QSYTTRTIQD TLNPKWNFNC
  1621  QFFIKDLYQD VLCLTLFDRD QFSPDDFLGR TEIPVAKIRT EQESKGPMTR RLLLHEVPTG
  1681  EVWVRFDLQL FEQKTLL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ITSN2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 69 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 69 nTPM
pancreas: 63 nTPM
lymph node: 50 nTPM
tonsil: 48 nTPM
thyroid gland: 47 nTPM
spleen: 41 nTPM

Single-cell type

esophageal apical cells: 1,103 nCPM
neutrophils: 747 nCPM
b-cells: 481 nCPM
neutrophil progenitors: 440 nCPM
urothelial cells: 397 nCPM
cdc: 377 nCPM

Immune cell

neutrophil: 113 nTPM
naive B-cell: 73 nTPM
basophil: 70 nTPM
memory B-cell: 69 nTPM
non-classical monocyte: 60 nTPM
eosinophil: 56 nTPM

Brain region

choroid plexus: 63 nTPM
thalamus: 60 nTPM
white matter: 57 nTPM
midbrain: 53 nTPM
medulla oblongata: 52 nTPM
cerebral cortex: 51 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.49
gnomAD pLI: 0
gnomAD missense Z: 1.12
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ITSN2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ITSN2 as an antibody target. Whether an autoantibody or antibody against ITSN2 could matter depends on whether native ITSN2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ITSN2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ITSN2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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