Seroatlas · Human Serome Atlas

ANKRD54

Ankyrin repeat domain-containing protein 54

Also known as: ANR54_HUMAN, LIAR

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6NXT1
Gene
ANKRD54
Ensembl
ENSG00000100124
Chromosome
22
Canonical length
300 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Microtubules,Midbody

OverviewNCBI Gene

Predicted to enable protein kinase regulator activity. Predicted to be involved in positive regulation of erythrocyte differentiation and regulation of intracellular signal transduction. Predicted to act upstream of or within nucleocytoplasmic transport and regulation of protein kinase activity. Predicted to be located in midbody. Predicted to be active in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

300 residues, UniProt reviewed canonical sequence.

>Q6NXT1|ANKRD54
     1  MAAAAGDADD EPRSGHSSSE GECAVAPEPL TDAEGLFSFA DFGSALGGGG AGLSGRASGG
    61  AQSPLRYLHV LWQQDAEPRD ELRCKIPAGR LRRAARPHRR LGPTGKEVHA LKRLRDSANA
   121  NDVETVQQLL EDGADPCAAD DKGRTALHFA SCNGNDQIVQ LLLDHGADPN QRDGLGNTPL
   181  HLAACTNHVP VITTLLRGGA RVDALDRAGR TPLHLAKSKL NILQEGHAQC LEAVRLEVKQ
   241  IIHMLREYLE RLGQHEQRER LDDLCTRLQM TSTKEQVDEV TDLLASFTSL SLQMQSMEKR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKRD54 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.42
Highest tissue expression
39 nTPM

Expression across tissuesHPA

Tissue

  • choroid plexus: 39 nTPM
  • testis: 32 nTPM
  • hypothalamus: 26 nTPM
  • cerebellum: 25 nTPM
  • cerebral cortex: 23 nTPM
  • amygdala: 23 nTPM

Single-cell type

  • epididymal efferent duct ciliated cells: 96 nCPM
  • fallopian tube ciliated cells: 78 nCPM
  • early primary spermatocytes: 75 nCPM
  • respiratory ciliated cells: 65 nCPM
  • endometrial ciliated cells: 63 nCPM
  • differentiating spermatogonia: 44 nCPM

Immune cell

  • NK-cell: 10 nTPM
  • non-classical monocyte: 9.4 nTPM
  • myeloid DC: 5.7 nTPM
  • intermediate monocyte: 5.5 nTPM
  • gdT-cell: 5.4 nTPM
  • MAIT T-cell: 5.4 nTPM

Brain region

  • choroid plexus: 34 nTPM
  • hypothalamus: 29 nTPM
  • midbrain: 26 nTPM
  • thalamus: 24 nTPM
  • medulla oblongata: 22 nTPM
  • hippocampal formation: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.83
gnomAD pLI
0
gnomAD missense Z
0.93
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANKRD54 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKRD54 as an antibody target. Whether an autoantibody or antibody against ANKRD54 could matter depends on whether native ANKRD54 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKRD54 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKRD54 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANKRD54. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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