IRF5

Protein identityUniProt · HPA

UniProt accession

Q13568

Gene

IRF5

Ensembl

ENSG00000128604

Chromosome

7

Canonical length

498 aa

Protein class

Disease related genes, Human disease related genes, Predicted intracellular proteins, Transcription factors

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]

Canonical amino-acid sequenceUniProt

498 residues, UniProt reviewed canonical sequence.

>Q13568|IRF5
     1  MNQSIPVAPT PPRRVRLKPW LVAQVNSCQY PGLQWVNGEK KLFCIPWRHA TRHGPSQDGD
    61  NTIFKAWAKE TGKYTEGVDE ADPAKWKANL RCALNKSRDF RLIYDGPRDM PPQPYKIYEV
   121  CSNGPAPTDS QPPEDYSFGA GEEEEEEEEL QRMLPSLSLT EDVKWPPTLQ PPTLRPPTLQ
   181  PPTLQPPVVL GPPAPDPSPL APPPGNPAGF RELLSEVLEP GPLPASLPPA GEQLLPDLLI
   241  SPHMLPLTDL EIKFQYRGRP PRALTISNPH GCRLFYSQLE ATQEQVELFG PISLEQVRFP
   301  SPEDIPSDKQ RFYTNQLLDV LDRGLILQLQ GQDLYAIRLC QCKVFWSGPC ASAHDSCPNP
   361  IQREVKTKLF SLEHFLNELI LFQKGQTNTP PPFEIFFCFG EEWPDRKPRE KKLITVQVVP
   421  VAARLLLEMF SGELSWSADS IRLQISNPDL KDRMVEQFKE LHHIWQSQQR LQPVAQAPPG
   481  AGLGVGQGPW PMHPAGMQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against IRF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.44

Highest tissue expression

16 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about IRF5.

Disease | AllUniProt

Conditions IRF5 is implicated in, by any mechanism.

• Inflammatory bowel disease 14 (IBD14) MIM:612245
• Systemic lupus erythematosus 10 (SLEB10) MIM:612251
• Rheumatoid arthritis (RA) MIM:180300

Disease | ImmuneIEDB

Conditions an epitope on IRF5 was assayed in.

• multiple sclerosis B and T cell
• infectious mononucleosis B and T cell
• peripheral nervous system disease B cell
• neuromyelitis optica B cell
• rheumatoid arthritis B cell
• systemic lupus erythematosus B cell
• systemic scleroderma B cell
• Sjogren's syndrome B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.6

gnomAD pLI

0.01

gnomAD missense Z

1.92

DepMap mean gene effect

0

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to muramyl dipeptide
• cellular response to peptidoglycan
• cellular response to virus
• cytokine-mediated signaling pathway
• defense response to virus
• immune system process
• inflammatory response
• innate immune response
• nucleotide-binding oligomerization domain containing 2 signaling pathway
• positive regulation of apoptotic process
• positive regulation of cytokine production involved in immune response
• positive regulation of interferon-alpha production
• positive regulation of interferon-beta production
• positive regulation of interleukin-12 production
• positive regulation of transcription by RNA polymerase II
• positive regulation of type I interferon production
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity, RNA polymerase II-specific
• identical protein binding
• protein kinase binding
• protein serine/threonine kinase binding
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• cytoplasm
• cytosol
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Interferon regulatory factor, DNA-binding domain
• SMAD/FHA domain superfamily
• SMAD-like domain superfamily
• Interferon regulatory factor-3
• Interferon regulatory factor, conserved site
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• Interferon regulatory factor transcription factor
• Interferon-regulatory factor 3

KeywordsUniProt

• Antiviral defense
• Cytoplasm
• DNA-binding
• Immunity
• Inflammatory response
• Innate immunity
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Systemic lupus erythematosus
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of IRF5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MYD88
• TASL
• TRAF6
• CREBBP
• MAVS
• SGTA