Seroatlas · Human Serome Atlas

HIRA

Protein HIRA

Also known as: DGCR1, HIRA_HUMAN, TUP1, TUPLE1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P54198
Gene
HIRA
Ensembl
ENSG00000100084
Chromosome
22
Canonical length
1017 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1017 residues, UniProt reviewed canonical sequence.

>P54198|HIRA
     1  MKLLKPTWVN HNGKPIFSVD IHPDGTKFAT GGQGQDSGKV VIWNMSPVLQ EDDEKDENIP
    61  KMLCQMDNHL ACVNCVRWSN SGMYLASGGD DKLIMVWKRA TYIGPSTVFG SSGKLANVEQ
   121  WRCVSILRNH SGDVMDVAWS PHDAWLASCS VDNTVVIWNA VKFPEILATL RGHSGLVKGL
   181  TWDPVGKYIA SQADDRSLKV WRTLDWQLET SITKPFDECG GTTHVLRLSW SPDGHYLVSA
   241  HAMNNSGPTA QIIEREGWKT NMDFVGHRKA VTVVKFNPKI FKKKQKNGSS AKPSCPYCCC
   301  AVGSKDRSLS VWLTCLKRPL VVIHELFDKS IMDISWTLNG LGILVCSMDG SVAFLDFSQD
   361  ELGDPLSEEE KSRIHQSTYG KSLAIMTEAQ LSTAVIENPE MLKYQRRQQQ QQLDQKSAAT
   421  REMGSATSVA GVVNGESLED IRKNLLKKQV ETRTADGRRR ITPLCIAQLD TGDFSTAFFN
   481  SIPLSGSLAG TMLSSHSSPQ LLPLDSSTPN SFGASKPCTE PVVAASARPA GDSVNKDSMN
   541  ATSTPAALSP SVLTTPSKIE PMKAFDSRFT ERSKATPGAP ALTSMTPTAV ERLKEQNLVK
   601  ELRPRDLLES SSDSDEKVPL AKASSLSKRK LELEVETVEK KKKGRPRKDS RLMPVSLSVQ
   661  SPAALTAEKE AMCLSAPALA LKLPIPSPQR AFTLQVSSDP SMYIEVENEV TVVGGVKLSR
   721  LKCNREGKEW ETVLTSRILT AAGSCDVVCV ACEKRMLSVF STCGRRLLSP ILLPSPISTL
   781  HCTGSYVMAL TAAATLSVWD VHRQVVVVKE ESLHSILAGS DMTVSQILLT QHGIPVMNLS
   841  DGKAYCFNPS LSTWNLVSDK QDSLAQCADF RSSLPSQDAM LCSGPLAIIQ GRTSNSGRQA
   901  ARLFSVPHVV QQETTLAYLE NQVAAALTLQ SSHEYRHWLL VYARYLVNEG FEYRLREICK
   961  DLLGPVHYST GSQWESTVVG LRKRELLKEL LPVIGQNLRF QRLFTECQEQ LDILRDK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HIRA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
53 nTPM

Expression across tissuesHPA

Tissue

  • thyroid gland: 53 nTPM
  • bone marrow: 25 nTPM
  • skeletal muscle: 22 nTPM
  • liver: 21 nTPM
  • parathyroid gland: 21 nTPM
  • cerebellum: 20 nTPM

Single-cell type

  • neutrophils: 57 nCPM
  • cardiomyocytes: 54 nCPM
  • myonuclei: 53 nCPM
  • hepatocytes: 52 nCPM
  • erythrocyte progenitors: 48 nCPM
  • early primary spermatocytes: 37 nCPM

Immune cell

  • basophil: 4.8 nTPM
  • naive B-cell: 4.5 nTPM
  • eosinophil: 3.3 nTPM
  • memory B-cell: 2.4 nTPM
  • NK-cell: 2.1 nTPM
  • plasmacytoid DC: 1.7 nTPM

Brain region

  • thalamus: 27 nTPM
  • amygdala: 23 nTPM
  • cerebral cortex: 22 nTPM
  • hippocampal formation: 22 nTPM
  • midbrain: 22 nTPM
  • basal ganglia: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.14
gnomAD pLI
1
gnomAD missense Z
3.71
DepMap mean gene effect
-0.65
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of HIRA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HIRA as an antibody target. Whether an autoantibody or antibody against HIRA could matter depends on whether native HIRA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HIRA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HIRA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HIRA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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