Seroatlas · Human Serome Atlas

FOXO4

Forkhead box protein O4

Also known as: AFX1, FOXO4_HUMAN, MLLT7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P98177
Gene
FOXO4
Ensembl
ENSG00000184481
Chromosome
X
Canonical length
505 aa
Protein class
Cancer-related genes, Disease related genes, Predicted intracellular proteins, RAS pathway related proteins, Transcription factors
Subcellular location
Nuclear speckles,Cytosol

OverviewNCBI Gene

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Canonical amino-acid sequenceUniProt

505 residues, UniProt reviewed canonical sequence.

>P98177|FOXO4
     1  MDPGNENSAT EAAAIIDLDP DFEPQSRPRS CTWPLPRPEI ANQPSEPPEV EPDLGEKVHT
    61  EGRSEPILLP SRLPEPAGGP QPGILGAVTG PRKGGSRRNA WGNQSYAELI SQAIESAPEK
   121  RLTLAQIYEW MVRTVPYFKD KGDSNSSAGW KNSIRHNLSL HSKFIKVHNE ATGKSSWWML
   181  NPEGGKSGKA PRRRAASMDS SSKLLRGRSK APKKKPSVLP APPEGATPTS PVGHFAKWSG
   241  SPCSRNREEA DMWTTFRPRS SSNASSVSTR LSPLRPESEV LAEEIPASVS SYAGGVPPTL
   301  NEGLELLDGL NLTSSHSLLS RSGLSGFSLQ HPGVTGPLHT YSSSLFSPAE GPLSAGEGCF
   361  SSSQALEALL TSDTPPPPAD VLMTQVDPIL SQAPTLLLLG GLPSSSKLAT GVGLCPKPLE
   421  APGPSSLVPT LSMIAPPPVM ASAPIPKALG TPVLTPPTEA ASQDRMPQDL DLDMYMENLE
   481  CDMDNIISDL MDEGEGLDFN FEPDP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FOXO4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.66
Highest tissue expression
110 nTPM

Expression across tissuesHPA

Tissue

  • placenta: 110 nTPM
  • skeletal muscle: 63 nTPM
  • midbrain: 61 nTPM
  • basal ganglia: 56 nTPM
  • amygdala: 54 nTPM
  • hippocampal formation: 53 nTPM

Single-cell type

  • cytotrophoblasts: 317 nCPM
  • syncytiotrophoblasts: 314 nCPM
  • migrating cytotrophoblasts: 202 nCPM
  • platelets: 21 nCPM
  • esophageal apical cells: 20 nCPM
  • neutrophils: 18 nCPM

Immune cell

  • eosinophil: 1 nTPM
  • neutrophil: 0.9 nTPM
  • naive CD8 T-cell: 0.4 nTPM
  • T-reg: 0.3 nTPM
  • total PBMC: 0.3 nTPM
  • gdT-cell: 0.2 nTPM

Brain region

  • white matter: 98 nTPM
  • basal ganglia: 96 nTPM
  • amygdala: 83 nTPM
  • medulla oblongata: 79 nTPM
  • midbrain: 76 nTPM
  • thalamus: 73 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.48
gnomAD pLI
0.81
gnomAD missense Z
1.65
DepMap mean gene effect
0.07
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of FOXO4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FOXO4 as an antibody target. Whether an autoantibody or antibody against FOXO4 could matter depends on whether native FOXO4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FOXO4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FOXO4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/FOXO4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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