Seroatlas · Human Serome Atlas

ERN1

Serine/threonine-protein kinase/endoribonuclease IRE1

Also known as: ERN1_HUMAN, IRE1, IRE1P

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O75460
Gene
ERN1
Ensembl
ENSG00000178607
Chromosome
17
Canonical length
977 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]

Canonical amino-acid sequenceUniProt

977 residues, UniProt reviewed canonical sequence.

>O75460|ERN1
     1  MPARRLLLLL TLLLPGLGIF GSTSTVTLPE TLLFVSTLDG SLHAVSKRTG SIKWTLKEDP
    61  VLQVPTHVEE PAFLPDPNDG SLYTLGSKNN EGLTKLPFTI PELVQASPCR SSDGILYMGK
   121  KQDIWYVIDL LTGEKQQTLS SAFADSLCPS TSLLYLGRTE YTITMYDTKT RELRWNATYF
   181  DYAASLPEDD VDYKMSHFVS NGDGLVVTVD SESGDVLWIQ NYASPVVAFY VWQREGLRKV
   241  MHINVAVETL RYLTFMSGEV GRITKWKYPF PKETEAKSKL TPTLYVGKYS TSLYASPSMV
   301  HEGVAVVPRG STLPLLEGPQ TDGVTIGDKG ECVITPSTDV KFDPGLKSKN KLNYLRNYWL
   361  LIGHHETPLS ASTKMLERFP NNLPKHRENV IPADSEKKSF EEVINLVDQT SENAPTTVSR
   421  DVEEKPAHAP ARPEAPVDSM LKDMATIILS TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ
   481  KELEKIQLLQ QQQQQLPFHP PGDTAQDGEL LDTSGPYSES SGTSSPSTSP RASNHSLCSG
   541  SSASKAGSSP SLEQDDGDEE TSVVIVGKIS FCPKDVLGHG AEGTIVYRGM FDNRDVAVKR
   601  ILPECFSFAD REVQLLRESD EHPNVIRYFC TEKDRQFQYI AIELCAATLQ EYVEQKDFAH
   661  LGLEPITLLQ QTTSGLAHLH SLNIVHRDLK PHNILISMPN AHGKIKAMIS DFGLCKKLAV
   721  GRHSFSRRSG VPGTEGWIAP EMLSEDCKEN PTYTVDIFSA GCVFYYVISE GSHPFGKSLQ
   781  RQANILLGAC SLDCLHPEKH EDVIARELIE KMIAMDPQKR PSAKHVLKHP FFWSLEKQLQ
   841  FFQDVSDRIE KESLDGPIVK QLERGGRAVV KMDWRENITV PLQTDLRKFR TYKGGSVRDL
   901  LRAMRNKKHH YRELPAEVRE TLGSLPDDFV CYFTSRFPHL LAHTYRAMEL CSHERLFQPY
   961  YFHEPPEPQP PVTPDAL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ERN1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
63 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 63 nTPM
  • adrenal gland: 27 nTPM
  • salivary gland: 14 nTPM
  • liver: 13 nTPM
  • bone marrow: 13 nTPM
  • stomach: 10 nTPM

Single-cell type

  • pdcs: 926 nCPM
  • neutrophils: 711 nCPM
  • adrenal cortex cells: 523 nCPM
  • plasma cells: 519 nCPM
  • pancreatic acinar cells: 327 nCPM
  • gonadotrophs: 319 nCPM

Immune cell

  • basophil: 18 nTPM
  • MAIT T-cell: 9 nTPM
  • plasmacytoid DC: 7.7 nTPM
  • gdT-cell: 4.9 nTPM
  • neutrophil: 4.8 nTPM
  • memory CD8 T-cell: 4.1 nTPM

Brain region

  • cerebral cortex: 12 nTPM
  • hypothalamus: 11 nTPM
  • cerebellum: 11 nTPM
  • choroid plexus: 10 nTPM
  • medulla oblongata: 9.2 nTPM
  • thalamus: 9.1 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ERN1.

Disease | ImmuneIEDB

Conditions an epitope on ERN1 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.29
gnomAD pLI
0.99
gnomAD missense Z
2.27
DepMap mean gene effect
-0.01
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ERN1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ERN1 as an antibody target. Whether an autoantibody or antibody against ERN1 could matter depends on whether native ERN1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ERN1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ERN1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ERN1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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