ENO1

Protein identityUniProt · HPA

UniProt accession

P06733

Gene

ENO1

Ensembl

ENSG00000074800

Chromosome

1

Canonical length

434 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Canonical amino-acid sequenceUniProt

434 residues, UniProt reviewed canonical sequence.

>P06733|ENO1
     1  MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK
    61  GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK
   121  AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE
   181  AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV
   241  VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD
   301  WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA
   361  QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK
   421  AKFAGRNFRN PLAK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENO1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.19

Highest tissue expression

1,003 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ENO1.

Disease | ImmuneIEDB

Conditions an epitope on ENO1 was assayed in.

• rheumatoid arthritis B and T cell
• multiple sclerosis B cell
• autoimmune thyroiditis B cell
• systemic lupus erythematosus B cell
• autoimmune disease of skin and connective tissue B cell
• Sjogren's syndrome B cell
• narcolepsy B cell
• peripheral nervous system disease B cell
• Timothy grass allergy T cell
• allergic disease T cell
• ovarian cancer T cell
• periodontal disease B cell

Disease | AutoantibodyPubMed

Conditions in which antibodies against ENO1 are reported. Each links to that disease's full target list.

• Hashimoto Disease 9
• Lupus Erythematosus, Systemic 9
• Encephalitis 8
• Lung Neoplasms 7
• Glomerulonephritis, Membranous 6
• Behcet Syndrome 5
• Brain Diseases 5
• Arthritis, Rheumatoid 4
• Lupus Nephritis 4
• Scleroderma, Systemic 4
• Cerebellar Ataxia 3
• Cryoglobulinemia 3
• Vasculitis 3

Showing 13 of 14 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for ENO1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.86

gnomAD pLI

0

gnomAD missense Z

0.84

DepMap mean gene effect

-0.38

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• canonical glycolysis
• gluconeogenesis
• glycolytic process
• negative regulation of cell growth
• negative regulation of DNA-templated transcription
• negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
• negative regulation of transcription by RNA polymerase II
• positive regulation of ATP biosynthetic process
• positive regulation of muscle contraction
• positive regulation of plasminogen activation
• response to virus

Molecular functions

• cadherin binding
• DNA-binding transcription repressor activity, RNA polymerase II-specific
• GTPase binding
• magnesium ion binding
• phosphopyruvate hydratase activity
• protein homodimerization activity
• RNA binding
• RNA polymerase II transcription regulatory region sequence-specific DNA binding
• transcription corepressor activity
• transcription corepressor binding

Cellular components

• cell cortex
• cell surface
• cytoplasm
• cytosol
• extracellular exosome
• extracellular space
• M band
• membrane
• nuclear outer membrane
• nucleus
• phosphopyruvate hydratase complex
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Enolase
• Enolase, conserved site
• Enolase, C-terminal TIM barrel domain
• Enolase, N-terminal
• Enolase-like, N-terminal
• Enolase-like, C-terminal domain superfamily
• Enolase, C-terminal TIM barrel domain
• Enolase, N-terminal domain

KeywordsUniProt

• Acetylation
• Alternative initiation
• Cell membrane
• Cytoplasm
• DNA-binding
• Glycolysis
• Hydroxylation
• Isopeptide bond
• Lyase
• Magnesium
• Membrane
• Metal-binding
• Nucleus
• Phosphoprotein
• Plasminogen activation
• Repressor
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of ENO1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CMTM6
• ENO4
• PGAM2
• PLG
• TRAPPC2B
• TRAPPC2
• ENO2
• ENO3
• INPPL1
• PCNA
• YWHAZ