ENO3

Protein identityUniProt · HPA

UniProt accession

P13929

Gene

ENO3

Ensembl

ENSG00000108515

Chromosome

17

Canonical length

434 aa

Protein class

Candidate cardiovascular disease genes, Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

434 residues, UniProt reviewed canonical sequence.

>P13929|ENO3
     1  MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK
    61  GVLKAVENIN NTLGPALLQK KLSVVDQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK
   121  AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE
   181  AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV
   241  VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD
   301  WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA
   361  QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK
   421  AIFAGRKFRN PKAK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENO3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.2

Highest tissue expression

13,830 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ENO3.

Disease | AllUniProt

Conditions ENO3 is implicated in, by any mechanism.

• Glycogen storage disease 13 (GSD13) MIM:612932

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 411 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Glycogen storage disease due to muscle beta-enolase deficiency

Disease | ImmuneIEDB

Conditions an epitope on ENO3 was assayed in.

• multiple sclerosis B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.69

gnomAD pLI

0

gnomAD missense Z

-0.93

DepMap mean gene effect

0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• canonical glycolysis
• gluconeogenesis
• glycolytic process

Molecular functions

• magnesium ion binding
• phosphopyruvate hydratase activity

Cellular components

• cytosol
• extracellular exosome
• extracellular space
• membrane
• phosphopyruvate hydratase complex
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Enolase
• Enolase, conserved site
• Enolase, C-terminal TIM barrel domain
• Enolase, N-terminal
• Enolase-like, N-terminal
• Enolase-like, C-terminal domain superfamily
• Enolase, C-terminal TIM barrel domain
• Enolase, N-terminal domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Glycogen storage disease
• Glycolysis
• Lyase
• Magnesium
• Metal-binding
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of ENO3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PNKD
• ENO1