ENO4
Enolase 4
Also known as: AC023283.3, C10orf134, ENO4_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- A6NNW6
- Gene
- ENO4
- Ensembl
- ENSG00000188316
- Chromosome
- 10
- Canonical length
- 625 aa
- Protein class
- Enzymes, Predicted intracellular proteins
- Subcellular location
- Vesicles,Cytosol
OverviewNCBI Gene
Predicted to enable phosphopyruvate hydratase activity. Predicted to be involved in glycolytic process. Predicted to act upstream of or within cilium organization and flagellated sperm motility. Predicted to be located in sperm principal piece. Predicted to be part of phosphopyruvate hydratase complex. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
625 residues, UniProt reviewed canonical sequence.
>A6NNW6|ENO4
1 MEEEGGGRSC GTTRELQKLK QQAMEYYREN DVPRRLEELL NSTFYLQPAD VYGHLANCFS
61 KLAKPPTICK IVGKDVLDGL GLPTLQVDIF CTIQNFPKNV CSVVISTHFE VHENALPELA
121 KAEEAERASA VSTAVQWVNS TITHELQGMA PSDQAEVDHL LRIFFASKVQ EDKGRKELEK
181 SLEYSTVPTP LPPVPPPPPP PPPTKKKGQK PGRKDTITEK PIAPAEPVEP VLSGSMAIGA
241 VSLAVAKACA MLLNKPLYLN IALLKHNQEQ PTTLSMPLLM VSLVSCGKSS SGKLNLMKEV
301 ICIPHPELTT KQGVEMLMEM QKHINKIIEM PSPPKAETKK GHDGSKRGQQ QITGKMSHLG
361 CLTINCDSIE QPLLLIQEIC ANLGLELGTN LHLAINCAGH ELMDYNKGKY EVIMGTYKNA
421 AEMVDLYVDL INKYPSIIAL IDPFRKEDSE QWDSIYHALG SRCYIIAGTA SKSISKLLEQ
481 GNISIPKSNG LIIKHTNQTT MSDLVEITNL IDSKKHITVF GSTEGESSDD SLVDLAVGLG
541 VRFIKLGGLS RGERVTKYNR LLTIEEELVQ NGTLGFKEEH TFFYFNEEAE KAAEALEAAA
601 AREPLVPTFP TQGVEESAET GASSGLocalizationUniProt · AlphaFold · HPA
Whether an antibody against ENO4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Unknown
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.32
- Highest tissue expression
- 5.2 nTPM
Expression across tissuesHPA
Tissue
- choroid plexus: 5.2 nTPM
- fallopian tube: 4.7 nTPM
- testis: 2.3 nTPM
- retina: 1.8 nTPM
- epididymis: 1.2 nTPM
- liver: 1.1 nTPM
Single-cell type
- epicardial cells: 355 nCPM
- ependymal cells: 255 nCPM
- cardiomyocytes: 116 nCPM
- oligodendrocytes: 116 nCPM
- fallopian tube ciliated cells: 107 nCPM
- respiratory ciliated cells: 105 nCPM
Immune cell
- plasmacytoid DC: 0.1 nTPM
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
Brain region
- white matter: 29 nTPM
- basal ganglia: 17 nTPM
- medulla oblongata: 16 nTPM
- choroid plexus: 16 nTPM
- midbrain: 16 nTPM
- thalamus: 14 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.49
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.28
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of ENO4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads ENO4 as an antibody target. Whether an autoantibody or antibody against ENO4 could matter depends on whether native ENO4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
ENO4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label ENO4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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