Seroatlas · Human Serome Atlas

ENAH

Protein enabled homolog

Also known as: ENAH_HUMAN, FLJ10773, MENA, NDPP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N8S7
Gene
ENAH
Ensembl
ENSG00000154380
Chromosome
1
Canonical length
591 aa
Protein class
Predicted intracellular proteins
Subcellular location
Plasma membrane,Focal adhesion sites,Cytosol
Quaternary structure
Homotetramer

OverviewNCBI Gene

This gene encodes a member of the enabled/ vasodilator-stimulated phosphoprotein. Members of this gene family are involved in actin-based motility. This protein is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility. Alternate splice variants of this gene have been correlated with tumor invasiveness in certain tissues and these variants may serve as prognostic markers. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2016]

Canonical amino-acid sequenceUniProt

591 residues, UniProt reviewed canonical sequence.

>Q8N8S7|ENAH
     1  MSEQSICQAR AAVMVYDDAN KKWVPAGGST GFSRVHIYHH TGNNTFRVVG RKIQDHQVVI
    61  NCAIPKGLKY NQATQTFHQW RDARQVYGLN FGSKEDANVF ASAMMHALEV LNSQETGPTL
   121  PRQNSQLPAQ VQNGPSQEEL EIQRRQLQEQ QRQKELERER LERERMERER LERERLERER
   181  LERERLEQEQ LERERQERER QERLERQERL ERQERLERQE RLDRERQERQ ERERLERLER
   241  ERQERERQEQ LEREQLEWER ERRISSAAAP ASVETPLNSV LGDSSASEPG LQAASQPAET
   301  PSQQGIVLGP LAPPPPPPLP PGPAQASVAL PPPPGPPPPP PLPSTGPPPP PPPPPLPNQV
   361  PPPPPPPPAP PLPASGFFLA SMSEDNRPLT GLAAAIAGAK LRKVSRMEDT SFPSGGNAIG
   421  VNSASSKTDT GRGNGPLPLG GSGLMEEMSA LLARRRRIAE KGSTIETEQK EDKGEDSEPV
   481  TSKASSTSTP EPTRKPWERT NTMNGSKSPV ISRRDSPRKN QIVFDNRSYD SLHRPKSTPL
   541  SQPSANGVQT EGLDYDRLKQ DILDEMRKEL TKLKEELIDA IRQELSKSNT A

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENAH can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.58
Highest tissue expression
38 nTPM

Expression across tissuesHPA

Tissue

  • blood vessel: 38 nTPM
  • smooth muscle: 38 nTPM
  • colon: 24 nTPM
  • urinary bladder: 19 nTPM
  • stomach: 19 nTPM
  • testis: 18 nTPM

Single-cell type

  • smooth muscle cells: 693 nCPM
  • sertoli cells: 615 nCPM
  • endometrial ciliated cells: 482 nCPM
  • pituitary stem cells: 469 nCPM
  • myonuclei: 452 nCPM
  • mucous neck cells: 452 nCPM

Immune cell

  • naive B-cell: 0.2 nTPM
  • basophil: 0.1 nTPM
  • neutrophil: 0.1 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • thalamus: 108 nTPM
  • hypothalamus: 99 nTPM
  • midbrain: 94 nTPM
  • medulla oblongata: 86 nTPM
  • amygdala: 86 nTPM
  • pons: 85 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ENAH.

Disease | ImmuneIEDB

Conditions an epitope on ENAH was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.17
gnomAD pLI
1
gnomAD missense Z
1.74
DepMap mean gene effect
-0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ENAH in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ENAH as an antibody target. Whether an autoantibody or antibody against ENAH could matter depends on whether native ENAH is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ENAH is annotated at the cell surface, where native ENAH is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ENAH as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ENAH. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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