Seroatlas · Human Serome Atlas

DENND4A

C-myc promoter-binding protein

Also known as: IRLB, MYCPBP, MYCPP_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q7Z401
Gene: DENND4A
Ensembl: ENSG00000174485
Chromosome: 15
Canonical length: 1863 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli rim,Mitotic chromosome

OverviewNCBI Gene

This gene encodes a DENN domain-containing protein that may function as a guanine nucleotide exchange factor that specifically activates ras-related protein Rab-10. This protein also contains a interferon stimulated response element-binding domain and may be involved in regulating the v-myc avian myelocytomatosis viral (MYC) oncogene. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8. [provided by RefSeq, Mar 2016]

Canonical amino-acid sequenceUniProt

1863 residues, UniProt reviewed canonical sequence.

>Q7Z401|DENND4A
     1  MIEDKGPRVA DYFVVAGLTD VSKPLEEEIH FNDACHKVAK PKEPITDVSV IIKSLGEEVP
    61  QDYICIDVTP TGLSADLNNG SLVGPQIYLC YRRGRDKPPL TDLGVLYDWK ERLKQGCEII
   121  QSTPYGRPAN ISGSTSSQRI YITYRRASEN MTQNTLAVTD ICIIIPSKGE SPPHTFCKVD
   181  KNLNNSMWGS AVYLCYKKSV AKTNTVSYKA GLICRYPQED YESFSLPESV PLFCLPMGAT
   241  IECWPSNSKY PLPVFSTFVL TGASAEKVYG AAIQFYEPYS EENLTEKQRL LLGLTSADGK
   301  SDSSKTIHTN KCICLLSHWP FFDAFRKFLT FLYRYSISGP HVLPIEKHIS HFMHKVPFPS
   361  PQRPRILVQL SPHDNLILSQ PVSSPLPLSG GKFSTLLQNL GPENAVTLLV FAVTEHKILI
   421  HSLRPSVLTS VTEALVSMIF PFHWPCPYVP LCPLALADVL SAPCPFIVGI DSRYFDLYDP
   481  PPDVSCVDVD TNTISQIGDK KNVAWKILPK KPCKNLMNTL NNLHQQLAKL QQRPRDDGLM
   541  DLAINDYDFN SGKRLHMIDL EIQEAFLFFM ASILKGYRSY LRPITQAPSE TATDAASLFA
   601  LQAFLRSRDR SHQKFYNMMT KTQMFIRFIE ECSFVSDKDA SLAFFDDCVD KVDMDKSGEV
   661  RLIELDESFK SEHTVFVTPP EIPHLPNGEE PPLQYSYNGF PVLRNNLFER PEGFLQAKKN
   721  KLPSKSSSPN SPLPMFRRTK QEIKSAHKIA KRYSSIPQMW SRCLLRHCYG LWFICLPAYV
   781  KVCHSKVRAL KTAYDVLKKM QSKKMDPPDE VCYRILMQLC GQYDQPVLAV RVLFEMQKAG
   841  IDPNAITYGY YNKAVLESTW PSRSRSGYFL WTKVRNVVLG VTQFKRALKK HAHLSQTTLS
   901  GGQSDLGYNS LSKDEVRRGD TSTEDIQEEK DKKGSDCSSL SESESTKGSA DCLPKLSYQN
   961  SSSIVRLTGT SNNSAGKISG ESMESTPELL LISSLEDTNE TRNIQSRCFR KRHKSDNETN
  1021  LQQQVVWGNR NRNLSGGVLM GFMLNRINQE ATPGDIVEKL GADAKILSNV ISKSTRPNTL
  1081  DIGKPPLRSK RDSLEKESSD DDTPFDGSNY LADKVDSPVI FDLEDLDSET DVSKAGCVAT
  1141  QNPKRIQRMN SSFSVKPFEK TDVATGFDPL SLLVAETEQQ QKEEEEEDED DSKSISTPSA
  1201  RRDLAEEIVM YMNNMSSPLT SRTPSIDLQR ACDDKLNKKS PPLVKACRRS SLPPNSPKPV
  1261  RLTKSKSYTK SEEKPRDRLW SSPAFSPTCP FREESQDTLT HSSPSFNLDT LLVPKLDVLR
  1321  NSMFTAGKGV AEKASKWYSR FTMYTTSSKD QSSDRTSLSS VGAQDSESTS LTDEDVCHEL
  1381  EGPISSQETS ATSGTKRIDL SRISLESSAS LEGSLSKFAL PGKSEVTSSF NASNTNIFQN
  1441  YAMEVLISSC SRCRTCDCLV HDEEIMAGWT ADDSNLNTTC PFCGNIFLPF LNIEIRDLRR
  1501  PGRYFLKSSP STENMHFPSS ISSQTRQSCI STSASGLDTS ALSVQGNFDL NSKSKLQENF
  1561  CTRSIQIPAN RSKTAMSKCP IFPMARSIST SGPLDKEDTG RQKLISTGSL PATLQGATDS
  1621  LGLEWHLPSP DPVTVPYLSP LVVWKELESL LENEGDHAIT VADFVDHHPI VFWNLVWYFR
  1681  RLDLPSNLPG LILSSEHCNK YSKIPRHCMS EDSKYVLIQM LWDNMKLHQD PGQPLYILWN
  1741  AHTQKYPMVH LLQKSDNSFN QELLKSMVKS IKMNDVYGPM SQILETLNKC PHFKRQRSLY
  1801  REILFLSLVA LGRENIDIDA FDKEYKMAYD RLTPSQVKST HNCDRPPSTG VMECRKTFGE
  1861  PYL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DENND4A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 55 nTPM

Expression across tissuesHPA

Tissue

retina: 55 nTPM
bone marrow: 32 nTPM
lymph node: 18 nTPM
tonsil: 17 nTPM
liver: 16 nTPM
pancreas: 15 nTPM

Single-cell type

mast cells: 1,001 nCPM
innate lymphoid cells: 908 nCPM
b-cells: 871 nCPM
sertoli cells: 707 nCPM
t-cells: 680 nCPM
neutrophils: 669 nCPM

Immune cell

naive B-cell: 6.2 nTPM
MAIT T-cell: 5.5 nTPM
memory B-cell: 5.4 nTPM
eosinophil: 5.3 nTPM
memory CD8 T-cell: 4.6 nTPM
gdT-cell: 3.6 nTPM

Brain region

cerebellum: 91 nTPM
choroid plexus: 53 nTPM
cerebral cortex: 44 nTPM
white matter: 44 nTPM
basal ganglia: 42 nTPM
hypothalamus: 40 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.28
gnomAD pLI: 1
gnomAD missense Z: 3.67
DepMap mean gene effect: -0.13
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DENND4A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DENND4A as an antibody target. Whether an autoantibody or antibody against DENND4A could matter depends on whether native DENND4A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DENND4A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DENND4A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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