Seroatlas · Human Serome Atlas

CHAF1A

Chromatin assembly factor 1 subunit A

Also known as: CAF-1, CAF1, CAF1A_HUMAN, CAF1B, CAF1P150, MGC71229, P150

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q13111
Gene
CHAF1A
Ensembl
ENSG00000167670
Chromosome
19
Canonical length
956 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

956 residues, UniProt reviewed canonical sequence.

>Q13111|CHAF1A
     1  MLEELECGAP GARGAATAMD CKDRPAFPVK KLIQARLPFK RLNLVPKGKA DDMSDDQGTS
    61  VQSKSPDLEA SLDTLENNCH VGSDIDFRPK LVNGKGPLDN FLRNRIETSI GQSTVIIDLT
   121  EDSNEQPDSL VDHNKLNSEA SPSREAINGQ REDTGDQQGL LKAIQNDKLA FPGETLSDIP
   181  CKTEEEGVGC GGAGRRGDSQ ECSPRSCPEL TSGPRMCPRK EQDSWSEAGG ILFKGKVPMV
   241  VLQDILAVRP PQIKSLPATP QGKNMTPESE VLESFPEEDS VLSHSSLSSP SSTSSPEGPP
   301  APPKQHSSTS PFPTSTPLRR ITKKFVKGST EKNKLRLQRD QERLGKQLKL RAEREEKEKL
   361  KEEAKRAKEE AKKKKEEEKE LKEKERREKR EKDEKEKAEK QRLKEERRKE RQEALEAKLE
   421  EKRKKEEEKR LREEEKRIKA EKAEITRFFQ KPKTPQAPKT LAGSCGKFAP FEIKEHMVLA
   481  PRRRTAFHPD LCSQLDQLLQ QQSGEFSFLK DLKGRQPLRS GPTHVSTRNA DIFNSDVVIV
   541  ERGKGDGVPE RRKFGRMKLL QFCENHRPAY WGTWNKKTAL IRARDPWAQD TKLLDYEVDS
   601  DEEWEEEEPG ESLSHSEGDD DDDMGEDEDE DDGFFVPHGY LSEDEGVTEE CADPENHKVR
   661  QKLKAKEWDE FLAKGKRFRV LQPVKIGCVW AADRDCAGDD LKVLQQFAAC FLETLPAQEE
   721  QTPKASKRER RDEQILAQLL PLLHGNVNGS KVIIREFQEH CRRGLLSNHT GSPRSPSTTY
   781  LHTPTPSEDA AIPSKSRLKR LISENSVYEK RPDFRMCWYV HPQVLQSFQQ EHLPVPCQWS
   841  YVTSVPSAPK EDSGSVPSTG PSQGTPISLK RKSAGSMCIT QFMKKRRHDG QIGAEDMDGF
   901  QADTEEEEEE EGDCMIVDVP DAAEVQAPCG AASGAGGGVG VDTGKATLTA SPLGAS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHAF1A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.62
Highest tissue expression
30 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 30 nTPM
  • thymus: 15 nTPM
  • testis: 14 nTPM
  • lymph node: 13 nTPM
  • skeletal muscle: 12 nTPM
  • skin: 10 nTPM

Single-cell type

  • pdcs: 165 nCPM
  • early primary spermatocytes: 156 nCPM
  • differentiating spermatogonia: 135 nCPM
  • erythrocyte progenitors: 132 nCPM
  • megakaryocyte progenitors: 90 nCPM
  • monocyte progenitors: 68 nCPM

Immune cell

  • plasmacytoid DC: 16 nTPM
  • eosinophil: 4.7 nTPM
  • basophil: 3.8 nTPM
  • classical monocyte: 2.3 nTPM
  • intermediate monocyte: 2.3 nTPM
  • myeloid DC: 2.1 nTPM

Brain region

  • cerebellum: 15 nTPM
  • cerebral cortex: 12 nTPM
  • white matter: 11 nTPM
  • amygdala: 10 nTPM
  • medulla oblongata: 9.9 nTPM
  • midbrain: 9.3 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CHAF1A.

Disease | GeneticClinVar

7 pathogenic / likely-pathogenic of 179 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.12
gnomAD pLI
1
gnomAD missense Z
1.85
DepMap mean gene effect
-1.1
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Chromatin assembly factor 1 p150 subunit, acidic region
  • Chromatin assembly factor 1 subunit A, dimerization domain
  • Chromatin assembly factor 1 subunit p150, N-terminal
  • Chromatin assembly factor 1 subunit p150, C-terminal
  • Chromatin assembly factor 1 complex p150 subunit, acidic region
  • Chromatin assembly factor 1 subunit A dimerization domain
  • CAF1 complex subunit p150, region binding to CAF1-p60 at C-term
  • CAF1 complex subunit p150, region binding to PCNA

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CHAF1A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHAF1A as an antibody target. Whether an autoantibody or antibody against CHAF1A could matter depends on whether native CHAF1A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHAF1A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHAF1A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CHAF1A. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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