Seroatlas · Human Serome Atlas

CHAF1B

Chromatin assembly factor 1 subunit B

Also known as: CAF-1, CAF1, CAF1A, CAF1B_HUMAN, CAF1P60, MPHOSPH7, MPP7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q13112
Gene
CHAF1B
Ensembl
ENSG00000159259
Chromosome
21
Canonical length
559 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

559 residues, UniProt reviewed canonical sequence.

>Q13112|CHAF1B
     1  MKVITCEIAW HNKEPVYSLD FQHGTAGRIH RLASAGVDTN VRIWKVEKGP DGKAIVEFLS
    61  NLARHTKAVN VVRFSPTGEI LASGGDDAVI LLWKVNDNKE PEQIAFQDED EAQLNKENWT
   121  VVKTLRGHLE DVYDICWATD GNLMASASVD NTAIIWDVSK GQKISIFNEH KSYVQGVTWD
   181  PLGQYVATLS CDRVLRVYSI QKKRVAFNVS KMLSGIGAEG EARSYRMFHD DSMKSFFRRL
   241  SFTPDGSLLL TPAGCVESGE NVMNTTYVFS RKNLKRPIAH LPCPGKATLA VRCCPVYFEL
   301  RPVVETGVEL MSLPYRLVFA VASEDSVLLY DTQQSFPFGY VSNIHYHTLS DISWSSDGAF
   361  LAISSTDGYC SFVTFEKDEL GIPLKEKPVL NMRTPDTAKK TKSQTHRGSS PGPRPVEGTP
   421  ASRTQDPSSP GTTPPQARQA PAPTVIRDPP SITPAVKSPL PGPSEEKTLQ PSSQNTKAHP
   481  SRRVTLNTLQ AWSKTTPRRI NLTPLKTDTP PSSVPTSVIS TPSTEEIQSE TPGDAQGSPP
   541  ELKRPRLDEN KGGTESLDP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHAF1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
15 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 15 nTPM
  • bone marrow: 6.4 nTPM
  • testis: 4 nTPM
  • thymus: 3.9 nTPM
  • cerebellum: 3.6 nTPM
  • tonsil: 3.3 nTPM

Single-cell type

  • respiratory deuterosomal cells: 40 nCPM
  • early primary spermatocytes: 30 nCPM
  • erythrocyte progenitors: 28 nCPM
  • differentiating spermatogonia: 23 nCPM
  • megakaryocyte progenitors: 22 nCPM
  • esophageal basal cells: 20 nCPM

Immune cell

  • T-reg: 2.2 nTPM
  • NK-cell: 1.7 nTPM
  • basophil: 1.4 nTPM
  • gdT-cell: 1.4 nTPM
  • MAIT T-cell: 1.2 nTPM
  • naive B-cell: 1.1 nTPM

Brain region

  • cerebellum: 8.2 nTPM
  • cerebral cortex: 7.1 nTPM
  • amygdala: 6.7 nTPM
  • basal ganglia: 6.2 nTPM
  • hippocampal formation: 5.6 nTPM
  • white matter: 5.6 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CHAF1B.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 87 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.44
gnomAD pLI
0.43
gnomAD missense Z
1.82
DepMap mean gene effect
-1.81
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CHAF1B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHAF1B as an antibody target. Whether an autoantibody or antibody against CHAF1B could matter depends on whether native CHAF1B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHAF1B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHAF1B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CHAF1B. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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