Seroatlas · Human Serome Atlas

ALKBH4

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4

Also known as: ALKB4_HUMAN, FLJ20013

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NXW9
Gene: ALKBH4
Ensembl: ENSG00000160993
Chromosome: 7
Canonical length: 302 aa
Protein class: Enzymes, Predicted intracellular proteins

OverviewNCBI Gene

Enables 2-oxoglutarate-dependent dioxygenase activity and actin binding activity. Involved in actomyosin structure organization and cleavage furrow ingression. Located in contractile ring and midbody. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

302 residues, UniProt reviewed canonical sequence.

>Q9NXW9|ALKBH4
     1  MAAAAAETPE VLRECGCKGI RTCLICERQR GSDPPWELPP AKTYRFIYCS DTGWAVGTEE
    61  SDFEGWAFPF PGVMLIEDFV TREEEAELVR LMDRDPWKLS QSGRRKQDYG PKVNFRKQKL
   121  KTEGFCGLPS FSREVVRRMG LYPGLEGFRP VEQCNLDYCP ERGSAIDPHL DDAWLWGERL
   181  VSLNLLSPTV LSMCREAPGS LLLCSAPSAA PEALVDSVIA PSRSVLCQEV EVAIPLPARS
   241  LLVLTGAARH QWKHAIHRRH IEARRVCVTF RELSAEFGPG GRQQELGQEL LRIALSFQGR
   301  PV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALKBH4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 11 nTPM
cerebral cortex: 7.1 nTPM
parathyroid gland: 6.5 nTPM
basal ganglia: 6.3 nTPM
spleen: 6.3 nTPM
amygdala: 6.2 nTPM

Single-cell type

cytotrophoblasts: 34 nCPM
differentiating spermatogonia: 28 nCPM
migrating cytotrophoblasts: 26 nCPM
endometrial luminal cells: 23 nCPM
esophageal basal cells: 22 nCPM
cone photoreceptor cells: 21 nCPM

Immune cell

non-classical monocyte: 9.4 nTPM
memory CD8 T-cell: 7.2 nTPM
NK-cell: 6.6 nTPM
MAIT T-cell: 5.6 nTPM
naive CD8 T-cell: 5.5 nTPM
plasmacytoid DC: 5.3 nTPM

Brain region

cerebellum: 10 nTPM
white matter: 8.5 nTPM
cerebral cortex: 7.7 nTPM
basal ganglia: 7.1 nTPM
thalamus: 6.8 nTPM
hypothalamus: 6.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.8
gnomAD pLI: 0.13
gnomAD missense Z: 0.56
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

actomyosin structure organization
demethylation
positive regulation of gene expression, epigenetic
cleavage furrow ingression

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily
Alpha-ketoglutarate-dependent dioxygenase alkB homologue 4

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ALKBH4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ALKBH4 as an antibody target. Whether an autoantibody or antibody against ALKBH4 could matter depends on whether native ALKBH4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ALKBH4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ALKBH4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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