Seroatlas · Human Serome Atlas

WEE1

Wee1-like protein kinase

Also known as: WEE1_HUMAN, WEE1A

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P30291
Gene
WEE1
Ensembl
ENSG00000166483
Chromosome
11
Canonical length
646 aa
Protein class
Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoli

OverviewNCBI Gene

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

646 residues, UniProt reviewed canonical sequence.

>P30291|WEE1
     1  MSFLSRQQPP PPRRAGAACT LRQKLIFSPC SDCEEEEEEE EEEGSGHSTG EDSAFQEPDS
    61  PLPPARSPTE PGPERRRSPG PAPGSPGELE EDLLLPGACP GADEAGGGAE GDSWEEEGFG
   121  SSSPVKSPAA PYFLGSSFSP VRCGGPGDAS PRGCGARRAG EGRRSPRPDH PGTPPHKTFR
   181  KLRLFDTPHT PKSLLSKARG IDSSSVKLRG SSLFMDTEKS GKREFDVRQT PQVNINPFTP
   241  DSLLLHSSGQ CRRRKRTYWN DSCGEDMEAS DYELEDETRP AKRITITESN MKSRYTTEFH
   301  ELEKIGSGEF GSVFKCVKRL DGCIYAIKRS KKPLAGSVDE QNALREVYAH AVLGQHSHVV
   361  RYFSAWAEDD HMLIQNEYCN GGSLADAISE NYRIMSYFKE AELKDLLLQV GRGLRYIHSM
   421  SLVHMDIKPS NIFISRTSIP NAASEEGDED DWASNKVMFK IGDLGHVTRI SSPQVEEGDS
   481  RFLANEVLQE NYTHLPKADI FALALTVVCA AGAEPLPRNG DQWHEIRQGR LPRIPQVLSQ
   541  EFTELLKVMI HPDPERRPSA MALVKHSVLL SASRKSAEQL RIELNAEKFK NSLLQKELKK
   601  AQMAKAAAEE RALFTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WEE1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.51
Highest tissue expression
59 nTPM

Expression across tissuesHPA

Tissue

  • endometrium: 59 nTPM
  • skin: 55 nTPM
  • liver: 54 nTPM
  • urinary bladder: 49 nTPM
  • gallbladder: 46 nTPM
  • esophagus: 44 nTPM

Single-cell type

  • endometrial glandular cells: 345 nCPM
  • ocular epithelial cells: 344 nCPM
  • urothelial cells: 300 nCPM
  • extravillous trophoblasts: 279 nCPM
  • endometrial luminal cells: 264 nCPM
  • breast secretory cells: 258 nCPM

Immune cell

  • memory B-cell: 8 nTPM
  • T-reg: 7.2 nTPM
  • eosinophil: 6 nTPM
  • naive B-cell: 4.7 nTPM
  • memory CD8 T-cell: 3 nTPM
  • NK-cell: 1.9 nTPM

Brain region

  • midbrain: 7 nTPM
  • pons: 6.3 nTPM
  • medulla oblongata: 5.1 nTPM
  • choroid plexus: 4.7 nTPM
  • hypothalamus: 4 nTPM
  • cerebellum: 3.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.17
gnomAD pLI
1
gnomAD missense Z
2.64
DepMap mean gene effect
-2.45
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of WEE1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WEE1 as an antibody target. Whether an autoantibody or antibody against WEE1 could matter depends on whether native WEE1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WEE1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label WEE1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/WEE1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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