Seroatlas · Human Serome Atlas

RUFY3

Protein RUFY3

Also known as: KIAA0871, RIPx, RUFY3_HUMAN, Singar1, ZFYVE30

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q7L099
Gene: RUFY3
Ensembl: ENSG00000018189
Chromosome: 4
Canonical length: 469 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

Canonical amino-acid sequenceUniProt

469 residues, UniProt reviewed canonical sequence.

>Q7L099|RUFY3
     1  MSALTPPTDM PTPTTDKITQ AAMETIYLCK FRVSMDGEWL CLRELDDISL TPDPEPTHED
    61  PNYLMANERM NLMNMAKLSI KGLIESALNL GRTLDSDYAP LQQFFVVMEH CLKHGLKAKK
   121  TFLGQNKSFW GPLELVEKLV PEAAEITASV KDLPGLKTPV GRGRAWLRLA LMQKKLSEYM
   181  KALINKKELL SEFYEPNALM MEEEGAIIAG LLVGLNVIDA NFCMKGEDLD SQVGVIDFSM
   241  YLKDGNSSKG TEGDGQITAI LDQKNYVEEL NRHLNATVNN LQAKVDALEK SNTKLTEELA
   301  VANNRIITLQ EEMERVKEES SYILESNRKG PKQDRTAEGQ ALSEARKHLK EETQLRLDVE
   361  KELEMQISMR QEMELAMKML EKDVCEKQDA LVSLRQQLDD LRALKHELAF KLQSSDLGVK
   421  QKSELNSRLE EKTNQMAATI KQLEQSEKDL VKQAKTLNSA ANKLIPKHH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RUFY3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 111 nTPM

Expression across tissuesHPA

Tissue

retina: 111 nTPM
cerebral cortex: 79 nTPM
spinal cord: 72 nTPM
hypothalamus: 65 nTPM
midbrain: 62 nTPM
hippocampal formation: 57 nTPM

Single-cell type

sertoli cells: 923 nCPM
adrenal cortex cells: 843 nCPM
somatotrophs: 673 nCPM
lactotrophs: 584 nCPM
corticotrophs: 561 nCPM
thyrotrophs: 559 nCPM

Immune cell

plasmacytoid DC: 6.6 nTPM
myeloid DC: 4.9 nTPM
non-classical monocyte: 4.9 nTPM
intermediate monocyte: 4.8 nTPM
memory B-cell: 3.6 nTPM
classical monocyte: 3.5 nTPM

Brain region

white matter: 219 nTPM
pons: 191 nTPM
hypothalamus: 174 nTPM
medulla oblongata: 166 nTPM
cerebral cortex: 160 nTPM
midbrain: 156 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.46
gnomAD pLI: 0.02
gnomAD missense Z: 2.48
DepMap mean gene effect: 0
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

dynactin binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

RUN domain
RUN domain superfamily
RUN and FYVE domain-containing protein 1-3
RUN domain
RUN and FYVE domain-containing protein 3, RUN domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RUFY3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RUFY3 as an antibody target. Whether an autoantibody or antibody against RUFY3 could matter depends on whether native RUFY3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RUFY3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RUFY3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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