RPS27A
Ubiquitin-ribosomal protein eS31 fusion protein
Also known as: RS27A_HUMAN, S27A, Uba80, UBCEP80
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P62979
- Gene
- RPS27A
- Ensembl
- ENSG00000143947
- Chromosome
- 2
- Canonical length
- 156 aa
- Protein class
- Predicted intracellular proteins, Ribosomal proteins
- Subcellular location
- Nucleoli,Endoplasmic reticulum,Cytosol,Acrosome,Equatorial segment
OverviewNCBI Gene
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]
Canonical amino-acid sequenceUniProt
156 residues, UniProt reviewed canonical sequence.
>P62979|RPS27A
1 MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN
61 IQKESTLHLV LRLRGGAKKR KKKSYTTPKK NKHKRKKVKL AVLKYYKVDE NGKISRLRRE
121 CPSDECGAGV FMASHFDRHY CGKCCLTYCF NKPEDKLocalizationUniProt · AlphaFold · HPA
Whether an antibody against RPS27A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.44
- Highest tissue expression
- 3,283 nTPM
Expression across tissuesHPA
Tissue
- ovary: 3,283 nTPM
- bone marrow: 2,417 nTPM
- breast: 2,347 nTPM
- pancreas: 2,265 nTPM
- cervix: 1,999 nTPM
- skin: 1,886 nTPM
Single-cell type
- esophageal suprabasal cells: 10,806 nCPM
- esophageal basal cells: 10,095 nCPM
- breast secretory cells: 8,456 nCPM
- extravillous trophoblasts: 8,042 nCPM
- ovarian stromal cells: 7,990 nCPM
- migrating cytotrophoblasts: 7,762 nCPM
Immune cell
- total PBMC: 8,236 nTPM
- naive CD4 T-cell: 5,693 nTPM
- naive CD8 T-cell: 4,760 nTPM
- memory B-cell: 4,746 nTPM
- memory CD4 T-cell: 4,519 nTPM
- MAIT T-cell: 4,278 nTPM
Brain region
- spinal cord: 434 nTPM
- thalamus: 389 nTPM
- white matter: 380 nTPM
- basal ganglia: 376 nTPM
- medulla oblongata: 348 nTPM
- hypothalamus: 345 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.37
- gnomAD pLI
- 0.92
- gnomAD missense Z
- 0.86
- DepMap mean gene effect
- -1.91
- DepMap dependency class
- pan
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cytoplasmic translation
- modification-dependent protein catabolic process
- protein ubiquitination
- translation
Molecular functions
- protein tag activity
- RNA binding
- structural constituent of ribosome
- ubiquitin protein ligase binding
- zinc ion binding
Cellular components
- cytoplasm
- cytosol
- cytosolic ribosome
- cytosolic small ribosomal subunit
- endocytic vesicle membrane
- endoplasmic reticulum
- endoplasmic reticulum membrane
- endosome membrane
- extracellular exosome
- extracellular space
- membrane
- mitochondrial outer membrane
- nucleolus
- nucleoplasm
- nucleus
- plasma membrane
- small-subunit processome
- synapse
- vesicle
Protein domainsUniProt · Pfam · InterPro
- Ubiquitin-like domain
- Zinc-binding ribosomal protein
- Ubiquitin conserved site
- Ubiquitin domain
- Ubiquitin-like domain superfamily
- Ubiquitin and ubiquitin-like
- Ubiquitin family
- Small ribosomal subunit protein eS31
- Small ribosomal subunit protein eS31 eukaryotic superfamily
- Ribosomal protein S27a
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of RPS27A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads RPS27A as an antibody target. Whether an autoantibody or antibody against RPS27A could matter depends on whether native RPS27A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
RPS27A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label RPS27A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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