Seroatlas · Human Serome Atlas

RNF8

E3 ubiquitin-protein ligase RNF8

Also known as: KIAA0646, RNF8_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O76064
Gene
RNF8
Ensembl
ENSG00000112130
Chromosome
6
Canonical length
485 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Canonical amino-acid sequenceUniProt

485 residues, UniProt reviewed canonical sequence.

>O76064|RNF8
     1  MGEPGFFVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS
    61  RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY
   121  EYEVTEEDWE TIYPCLSPKN DQMIEKNKEL RTKRKFSLDE LAGPGAEGPS NLKSKINKVS
   181  CESGQPVKSQ GKGEVASTPS DNLDPKLTAL EPSKTTGAPI YPGFPKVTEV HHEQKASNSS
   241  ASQRSLQMFK VTMSRILRLK IQMQEKHEAV MNVKKQTQKG NSKKVVQMEQ ELQDLQSQLC
   301  AEQAQQQARV EQLEKTFQEE EQHLQGLEIA QGEKDLKQQL AQALQEHWAL MEELNRSKKD
   361  FEAIIQAKNK ELEQTKEEKE KMQAQKEEVL SHMNDVLENE LQCIICSEYF IEAVTLNCAH
   421  SFCSYCINEW MKRKIECPIC RKDIKSKTYS LVLDNCINKM VNNLSSEVKE RRIVLIRERK
   481  AKRLF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
14 nTPM

Expression across tissuesHPA

Tissue

  • choroid plexus: 14 nTPM
  • cerebellum: 14 nTPM
  • testis: 11 nTPM
  • esophagus: 10 nTPM
  • cerebral cortex: 10 nTPM
  • tongue: 9.9 nTPM

Single-cell type

  • sertoli cells: 117 nCPM
  • esophageal apical cells: 99 nCPM
  • megakaryocytes: 99 nCPM
  • esophageal suprabasal cells: 81 nCPM
  • endometrial luminal cells: 74 nCPM
  • platelets: 73 nCPM

Immune cell

  • gdT-cell: 9.5 nTPM
  • T-reg: 9.2 nTPM
  • memory CD8 T-cell: 9.1 nTPM
  • MAIT T-cell: 8.8 nTPM
  • non-classical monocyte: 8.8 nTPM
  • memory B-cell: 8.1 nTPM

Brain region

  • cerebellum: 18 nTPM
  • cerebral cortex: 17 nTPM
  • thalamus: 16 nTPM
  • midbrain: 15 nTPM
  • hypothalamus: 15 nTPM
  • pons: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.18
gnomAD pLI
1
gnomAD missense Z
2.16
DepMap mean gene effect
-0.57
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF8 as an antibody target. Whether an autoantibody or antibody against RNF8 could matter depends on whether native RNF8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF8. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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